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哈皮miao銀蟲 (小有名氣)
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[求助]
一個英文實(shí)驗(yàn)方法,求解釋!
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Gelatin solution(26wt%) was mixed with GA solution (1wt%) with the gelatin/GA volume ratio 7.5/1.0 at 45 ℃ for gelation in a polypropylene tube and allowed to react for 2min. In the case of albumin gel, 45wt% albumin solution was mixed with 10wt% GA solution with the albumin/GA volume ratio 4.0/1.0. After addition of 5mLPBS(-)solution to the gel (1g) and incubation at 37℃ for predetermined durations, the supernatant(extract) was collected and the same amount of new PBS(-) was added, followed by extraction. Release of the unreacted aldehyde from the gelatin gel to each of PBS(-)was determined with the MBTH method. 我理解的是: 26wt%的明膠溶液與1wt%的戊二醛溶液按體積比7.5::1.0在45℃條件下混合,塑料管內(nèi)反應(yīng)2min,制成1g膠;45wt%的牛血清白蛋白溶液與10wt%戊二醛溶液按體積比4.0:1.0按上述方法制成1g膠。 然后在塑料管內(nèi)加入5mlPBS,37℃培養(yǎng)預(yù)定時間(如1,2,3,4,5h)后,收集上清液,用MBTH方法測量5ml上清液中未反應(yīng)醛的釋放量。同時再在這個塑料管內(nèi)加入5mlPBS,再培養(yǎng)預(yù)定時間后,收集上清液,MBTH方法測量5ml上清液中未反應(yīng)醛的釋放量。 如果時間間隔均為1h,那個對于同1g膠來說,每個5mlPBS測得的醛含量為每1小時內(nèi)的釋放量,如果我想得到前2,3,4,5...小時內(nèi)的醛釋放量,就得把每個值相加,這樣理解對嗎?如果對的話,相加得到的值誤差不會不太大?謝謝各位解答,不勝感激! |

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