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以白色棉隴棉2號為對照,對綠色棉隴綠棉3號,棕色棉BC05進行田間卡那霉素(Kan)葉片涂抹篩選轉(zhuǎn)基因植株梯度試驗,比較了兩個彩色棉品種田間篩選表現(xiàn)(圖)、篩選濃度及適宜觀察時間,并對篩選的抗性植株進行了PCR檢測驗證。結果表明:兩個彩色棉品種在田間篩選表現(xiàn)、篩選濃度及適宜觀察時間上均有差異,與對照相比,隴綠棉3號田間表現(xiàn)最不明顯,Kan篩選濃度為3.0~7.0 g•L-1,觀察時間為5~16 d ;BC05田間表現(xiàn)較為明顯,Kan篩選濃度為3.0~7.0 g•L-1,觀察時間為4~15 d,經(jīng)PCR檢測驗證,兩個彩色棉品種Kan陽性植株篩選準確率達96.1 %,本次試驗研究,初步探索了Kan葉片涂抹篩選法在轉(zhuǎn)基因彩色棉植株篩選上的應用,對轉(zhuǎn)基因彩色棉的選育有參考價值。 我寫作少,這篇內(nèi)容我想發(fā) 分子植物育種 ,不知道如何 |
| Contrast, white cotton Gansu cotton on the 2nd green cotton on the 3rd green cotton Gansu , brown cotton BC05 field kanamycin (Kan) leaves smear screening transgenic plants gradient test to compare the screening performance of the two colored cotton varieties field ( figure), the concentration of screening resistant plants and suitable for the observation time , and screening PCR assay validation . The results showed that : two colored cotton varieties screened in the field performance of screening on the concentration and the appropriate observation time were different , compared with the control , Gansu green cotton field performance of the most obvious , Kan, screening concentration of 3.0 ~ 7.0 g • L -1, the observation time of 5 to 16 days ; BC05 field performance of the more obvious , Kan, screening concentration of 3.0 ~~ 7.0 g • L -1 , observation time was 4 for 15 days , were detected by PCR |

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