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haiyanqian木蟲 (小有名氣)
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[求助]
同源模建的一些問題
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| 我想問一下,同源模建時是如何確定我的結(jié)構(gòu)保守區(qū),還有就是根據(jù)什么來定義helix的長度。看文獻都是直接那張序列比對圖就出來了。在用Clustal進行序列比對時,那個下面有“:”和“."分別是表示什么意思呀?哪位知道的幫忙回答一下吧,非常感謝! |
木蟲 (小有名氣)
鐵蟲 (初入文壇)
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clustal 的help里面有關(guān)于clustal系列版本的好幾篇參考文獻,比如說關(guān)于clustalX的Thompson,J.D., Gibson,T.J., Plewniak,F., Jeanmougin,F. and Higgins,D.G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25:4876-4882. 里邊就有提到:The colouring of residues takes place according to physicochemical criteria highlighting conserved positions in the sequences. A consensus line is also displayed below the alignment with the following symbols denoting the degree of conservation observed in each column: ‘*’ (identical residues in all sequences), ‘:’ (highly conserved column), ‘.’ (weakly conserved column).(摘自原文)去看看吧。 |

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