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issaclai木蟲 (小有名氣)
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[求助]
求助:審稿意見中有些不明白的地方,請教各位了
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我的論文中只寫了培養(yǎng)基,每個方法里面寫了細(xì)菌培養(yǎng)好后離心洗滌的處理過程。但審稿人提出:How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? 不知道該怎么回答,特別是里面的pre culture 和main culture是什么意思,請教各位了! |
木蟲 (小有名氣)
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能告訴我您的QQ號嗎,我不是學(xué)生物的,所以很多都不懂,想具體請教您關(guān)于這句話的意思! 我做的是大腸桿菌,培養(yǎng)基是買的,然后配置的, 我的文章中是這么寫的Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃. |
木蟲 (小有名氣)
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我的文章中是這么寫的: Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃. 審稿意見是: Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient. |
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這個問題人家是說你對培養(yǎng)物的制備說的太不詳細(xì)了。Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient. 主要是缺少細(xì)節(jié):培養(yǎng)物的如何得到的?培養(yǎng)條件樓主沒提到,是多少溫度,多少時間等等。種子培養(yǎng)還是發(fā)酵培養(yǎng)。離心過程的條件,包括轉(zhuǎn)速、時間等。細(xì)胞計數(shù)的方法要涉及到。 |
木蟲 (小有名氣)
金蟲 (著名寫手)
木蟲 (小有名氣)
木蟲 (小有名氣)
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