| 5 | 1/1 | 返回列表 |
| 查看: 1045 | 回復(fù): 8 | |||
| 當(dāng)前只顯示滿足指定條件的回帖,點擊這里查看本話題的所有回帖 | |||
issaclai木蟲 (小有名氣)
|
[求助]
求助:審稿意見中有些不明白的地方,請教各位了
|
||
|
我的論文中只寫了培養(yǎng)基,每個方法里面寫了細(xì)菌培養(yǎng)好后離心洗滌的處理過程。但審稿人提出:How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? 不知道該怎么回答,特別是里面的pre culture 和main culture是什么意思,請教各位了! |
木蟲 (小有名氣)
|
能告訴我您的QQ號嗎,我不是學(xué)生物的,所以很多都不懂,想具體請教您關(guān)于這句話的意思! 我做的是大腸桿菌,培養(yǎng)基是買的,然后配置的, 我的文章中是這么寫的Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃. |
木蟲 (小有名氣)
|
我的文章中是這么寫的: Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃. 審稿意見是: Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient. |
|
這個問題人家是說你對培養(yǎng)物的制備說的太不詳細(xì)了。Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient. 主要是缺少細(xì)節(jié):培養(yǎng)物的如何得到的?培養(yǎng)條件樓主沒提到,是多少溫度,多少時間等等。種子培養(yǎng)還是發(fā)酵培養(yǎng)。離心過程的條件,包括轉(zhuǎn)速、時間等。細(xì)胞計數(shù)的方法要涉及到。 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 求生物學(xué)調(diào)劑 +8 | 15172915737 2026-04-01 | 8/400 |
|
|---|---|---|---|---|
|
[考研] 生物學(xué)327,求調(diào)劑 +5 | 書上的梅子 2026-04-01 | 6/300 |
|
|
[考研] 265求調(diào)劑 +4 | 林深溫瀾 2026-04-01 | 6/300 |
|
|
[考研] 生物與醫(yī)藥考研調(diào)劑 +5 | 鐵憨憨123425 2026-03-31 | 5/250 |
|
|
[考研] 311求調(diào)劑 +11 | 藍(lán)月亮亮 2026-03-30 | 11/550 |
|
|
[考研] 調(diào)劑推薦 +9 | 清酒714 2026-03-26 | 10/500 |
|
|
[考研] 085601一志愿中山大學(xué)深圳材料工程330求調(diào)劑 +6 | pipiver 2026-03-30 | 6/300 |
|
|
[考研] 0703化學(xué)/290求調(diào)劑/本科經(jīng)歷豐富/工科也可 +14 | 丹青奶蓋 2026-03-26 | 16/800 |
|
|
[考研] 一志愿中國科學(xué)院大學(xué)265求調(diào)劑 +8 | 恬淡ye 2026-03-31 | 9/450 |
|
|
[考研] 一志愿同濟(jì)大學(xué)323分(080500)求調(diào)劑 +4 | yikeniu 2026-04-01 | 4/200 |
|
|
[考研] 311求調(diào)劑一志愿合肥工業(yè)大學(xué) +14 | 秋二十二 2026-03-30 | 14/700 |
|
|
[考研] 材料專業(yè)求調(diào)劑 +7 | 月月鳥木 2026-04-01 | 7/350 |
|
|
[碩博家園] 博一被送出聯(lián)培感覺不適應(yīng)怎么辦 +3 | 全村的狗 2026-03-31 | 3/150 |
|
|
[考研] 求0861交通運輸專碩or材料專碩調(diào)劑 +4 | 勒布朗@ 2026-03-31 | 4/200 |
|
|
[考研] 070300化學(xué)專業(yè)279調(diào)劑 +10 | 哈哈哈^_^ 2026-03-31 | 10/500 |
|
|
[考研] 315求調(diào)劑 +6 | akie... 2026-03-28 | 7/350 |
|
|
[考研] 276求調(diào)劑 +3 | 趙久華 2026-03-29 | 3/150 |
|
|
[考研] 一志愿中海洋320化學(xué)工程與技術(shù)學(xué)碩求調(diào)劑 +8 | 披星河 2026-03-30 | 8/400 |
|
|
[考研] 哈爾濱工業(yè)大學(xué)材料與化工專碩378求調(diào)劑 +3 | 塔比烏斯 2026-03-30 | 3/150 |
|
|
[考研] 08開頭275求調(diào)劑 +4 | 拉誰不重要 2026-03-26 | 4/200 |
|