| 5 | 1/1 | 返回列表 |
| 查看: 1047 | 回復(fù): 8 | |||
| 當(dāng)前只顯示滿足指定條件的回帖,點(diǎn)擊這里查看本話題的所有回帖 | |||
issaclai木蟲 (小有名氣)
|
[求助]
求助:審稿意見(jiàn)中有些不明白的地方,請(qǐng)教各位了
|
||
|
我的論文中只寫了培養(yǎng)基,每個(gè)方法里面寫了細(xì)菌培養(yǎng)好后離心洗滌的處理過(guò)程。但審稿人提出:How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? 不知道該怎么回答,特別是里面的pre culture 和main culture是什么意思,請(qǐng)教各位了! |
木蟲 (小有名氣)
|
我的文章中是這么寫的: Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃. 審稿意見(jiàn)是: Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient. |
木蟲 (小有名氣)
|
能告訴我您的QQ號(hào)嗎,我不是學(xué)生物的,所以很多都不懂,想具體請(qǐng)教您關(guān)于這句話的意思! 我做的是大腸桿菌,培養(yǎng)基是買的,然后配置的, 我的文章中是這么寫的Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃. |
|
這個(gè)問(wèn)題人家是說(shuō)你對(duì)培養(yǎng)物的制備說(shuō)的太不詳細(xì)了。Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient. 主要是缺少細(xì)節(jié):培養(yǎng)物的如何得到的?培養(yǎng)條件樓主沒(méi)提到,是多少溫度,多少時(shí)間等等。種子培養(yǎng)還是發(fā)酵培養(yǎng)。離心過(guò)程的條件,包括轉(zhuǎn)速、時(shí)間等。細(xì)胞計(jì)數(shù)的方法要涉及到。 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 材料與化工306分找調(diào)劑 +10 | 滄海輕舟e 2026-04-02 | 10/500 |
|
|---|---|---|---|---|
|
[考研] 一志愿上海海洋大學(xué)083200食品學(xué)碩,求調(diào)劑,接受其他專業(yè) +6 | what張 2026-04-01 | 7/350 |
|
|
[考研] 材料340分調(diào)劑 +6 | 夏夜晚風(fēng)_long 2026-04-02 | 8/400 |
|
|
[考研] 285求調(diào)劑 +14 | AZMK 2026-04-02 | 14/700 |
|
|
[考研] 283求調(diào)劑 +3 | jiouuu 2026-04-02 | 4/200 |
|
|
[考研] 266求調(diào)劑 +4 | 學(xué)員97LZgn 2026-04-02 | 4/200 |
|
|
[考研] 322求調(diào)劑 +5 | 熹僖XX 2026-03-31 | 6/300 |
|
|
[考研] 【求調(diào)劑】新能源材料本科,一志愿211,初試321 +6 | 求調(diào)劑學(xué)校, 2026-04-02 | 6/300 |
|
|
[考研] 一志愿346上海大學(xué)生物學(xué) +3 | 上海大學(xué)346調(diào)劑 2026-04-01 | 3/150 |
|
|
[考研] 08工科275分求調(diào)劑 +13 | AaAa7420 2026-03-31 | 13/650 |
|
|
[考研] 085410 一志愿211 22408分?jǐn)?shù)359求調(diào)劑 +3 | 123456789qw 2026-03-31 | 4/200 |
|
|
[考研] 調(diào)劑 +3 | 好好讀書。 2026-04-01 | 3/150 |
|
|
[碩博家園] 考研調(diào)劑 +5 | 駱駝男人 2026-04-01 | 5/250 |
|
|
[考研] 環(huán)境工程調(diào)劑 +9 | hyzzzzzzz. 2026-04-01 | 9/450 |
|
|
[考研] 333求調(diào)劑 +4 | 阿科逸 2026-03-31 | 4/200 |
|
|
[考研] 考研調(diào)劑 +9 | 小蠟新筆 2026-03-29 | 10/500 |
|
|
[考研] 本2一志愿C9-333分,材料科學(xué)與工程,求調(diào)劑 +9 | 升升不降 2026-03-31 | 9/450 |
|
|
[考研] 江蘇蘇北高校誠(chéng)邀調(diào)劑同學(xué) +3 | zzll406 2026-03-31 | 3/150 |
|
|
[考研] 求調(diào)劑 +8 | 11ggg 2026-03-30 | 8/400 |
|
|
[考研] 環(huán)境科學(xué)與工程334分求調(diào)劑 +6 | 王一一依依 2026-03-30 | 8/400 |
|