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[求助]
生物的達(dá)人們,請(qǐng)教一個(gè)關(guān)于檢測(cè)對(duì)象的問(wèn)題!
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生物的前輩們好,小女子一事相求,本人有一種新的熒光檢測(cè)方法,老板讓與生物學(xué)知識(shí)結(jié)合,作一篇相關(guān)檢測(cè)的文章,但苦于生物知識(shí)的缺乏,沒(méi)有好的想法,所以懇求大家給與指導(dǎo)點(diǎn)播,給些建議,我的熒光材料與——OH,——COOH,——NH2都具有一定的結(jié)合能力~~~類似的有人做過(guò)檢測(cè)DNA甲基化水平和蛋白質(zhì)磷酸化水平的檢測(cè),相關(guān)的還懇請(qǐng)大家?guī)蛦,出點(diǎn)主意,王能不辭吝教,小女子不勝感激!謝啦先~~![]() ![]() |

至尊木蟲(chóng) (著名寫手)
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biotin用的比較多 http://en.wikipedia.org/wiki/Biotin Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays.[34] Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10−14 mol/l to 10−15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction. |
金蟲(chóng) (小有名氣)

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