| 10 | 1/1 | 返回列表 |
| 查看: 575 | 回復(fù): 9 | ||
淺笑包包新蟲 (小有名氣)
|
[求助]
assay of ribonuclease activity
|
|
Ten microliters of protein (0.25 mgÆml)1) and 10 ll of saffron leaf total RNA (10 lgÆll)1) were mixed with 100 ll 50 mm MES (pH 6.0) and incubated at 50 C for 30 min. The reaction was stopped by adding 1 ml of ice-cold 4 m LiCl to the mixture, and incubated in ice for 15 min. Insoluble leaf total RNA was removed by centrifugation (10,000 g) for 15 min at 4 C. The supernatant containing soluble leaf total RNA was quantified by UV absorbance at 260 nm in a spectrophotometer (Lan Optics). One unit of enzyme was defined as the amount of protein causing an increase of absorption of 1.0 after 30 min of incubation. The positive and negative controls were RNase A and the heated CsPR10 (100 C for 10 min), respectively. 上述來自文獻(xiàn) 請(qǐng)問是什么意思?為什么要把蛋白質(zhì)與RNA融合?融合后又如何表達(dá)出了它的核糖核苷酸活性? |

鐵桿木蟲 (職業(yè)作家)
新蟲 (小有名氣)

鐵桿木蟲 (職業(yè)作家)
新蟲 (小有名氣)

鐵桿木蟲 (職業(yè)作家)
新蟲 (小有名氣)

鐵桿木蟲 (職業(yè)作家)
木蟲 (著名寫手)
清風(fēng)小木蟲
新蟲 (小有名氣)

| 10 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 材料求調(diào)劑 一志愿哈工大總分298分,前三科223分 +3 | dongfang59 2026-03-27 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 266分,求材料冶金能源化工等調(diào)劑 +5 | 哇呼哼呼哼 2026-03-27 | 7/350 |
|
|
[考研] 070300化學(xué)求調(diào)劑 +4 | 起個(gè)名咋這么難 2026-03-27 | 4/200 |
|
|
[考研] 085600材料與化工調(diào)劑 +10 | A-哆啦Z夢(mèng) 2026-03-23 | 16/800 |
|
|
[考研] 考研化學(xué)308分求調(diào)劑 +10 | 你好明天你好 2026-03-23 | 12/600 |
|
|
[考研] 一志愿北京化工大學(xué)材料與化工 264分各科過A區(qū)國家線 +10 | 哈哈157349 2026-03-21 | 10/500 |
|
|
[考研] 348求調(diào)劑 +4 | 小懶蟲不懶了 2026-03-27 | 5/250 |
|
|
[考研] 0703化學(xué)/290求調(diào)劑/本科經(jīng)歷豐富/工科也可 +7 | 丹青奶蓋 2026-03-26 | 8/400 |
|
|
[考研] 286求調(diào)劑 +4 | lim0922 2026-03-26 | 4/200 |
|
|
[考研] 317求調(diào)劑 +7 | 蛋黃咸肉粽 2026-03-26 | 7/350 |
|
|
[考研] 349求調(diào)劑 +5 | 杰斯塔里斯 2026-03-21 | 5/250 |
|
|
[考研]
|
平樂樂樂 2026-03-26 | 4/200 |
|
|
[考研] 一志愿 南京郵電大學(xué) 288分 材料考研 求調(diào)劑 +3 | jl0720 2026-03-26 | 3/150 |
|
|
[考研] 299求調(diào)劑 +4 | 15188958825 2026-03-25 | 4/200 |
|
|
[考研] 一志愿中南大學(xué)化學(xué)學(xué)碩0703總分337求調(diào)劑 +7 | niko- 2026-03-22 | 7/350 |
|
|
[考研] 284求調(diào)劑 +15 | Zhao anqi 2026-03-22 | 15/750 |
|
|
[考研] 化學(xué)調(diào)劑 +6 | yzysaa 2026-03-21 | 6/300 |
|
|
[考研] B區(qū)考研調(diào)劑 +4 | yqdszhdap- 2026-03-22 | 5/250 |
|
|
[考研] 335求調(diào)劑 +4 | yuyu宇 2026-03-23 | 5/250 |
|
|
[考研] 269求調(diào)劑 +4 | 我想讀研11 2026-03-23 | 4/200 |
|