| 5 | 1/1 | 返回列表 |
| 查看: 577 | 回復: 9 | ||
| 當前只顯示滿足指定條件的回帖,點擊這里查看本話題的所有回帖 | ||
淺笑包包新蟲 (小有名氣)
|
[求助]
assay of ribonuclease activity
|
|
|
Ten microliters of protein (0.25 mgÆml)1) and 10 ll of saffron leaf total RNA (10 lgÆll)1) were mixed with 100 ll 50 mm MES (pH 6.0) and incubated at 50 C for 30 min. The reaction was stopped by adding 1 ml of ice-cold 4 m LiCl to the mixture, and incubated in ice for 15 min. Insoluble leaf total RNA was removed by centrifugation (10,000 g) for 15 min at 4 C. The supernatant containing soluble leaf total RNA was quantified by UV absorbance at 260 nm in a spectrophotometer (Lan Optics). One unit of enzyme was defined as the amount of protein causing an increase of absorption of 1.0 after 30 min of incubation. The positive and negative controls were RNase A and the heated CsPR10 (100 C for 10 min), respectively. 上述來自文獻 請問是什么意思?為什么要把蛋白質與RNA融合?融合后又如何表達出了它的核糖核苷酸活性? |

鐵桿木蟲 (職業(yè)作家)
鐵桿木蟲 (職業(yè)作家)
新蟲 (小有名氣)

新蟲 (小有名氣)

| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿211 初試270分 求調劑 +8 | 谷雨上岸 2026-03-23 | 9/450 |
|
|---|---|---|---|---|
|
[考研] 0703 化學 求調劑,一志愿山東大學 342 分 +4 | Shern—- 2026-03-28 | 4/200 |
|
|
[考研] 321求調劑 +6 | 璞玉~~ 2026-03-25 | 7/350 |
|
|
[考研] 復試調劑 +3 | raojunqi0129 2026-03-28 | 3/150 |
|
|
[考研] 070300求調劑306分 +4 | 26要上岸 2026-03-27 | 4/200 |
|
|
[考研] 調劑 +3 | 好好讀書。 2026-03-28 | 3/150 |
|
|
[考研] 277跪求調劑 +5 | 1915668 2026-03-27 | 9/450 |
|
|
[考研] 085405 考的11408求各位老師帶走 +3 | Qiu學ing 2026-03-28 | 3/150 |
|
|
[考研] 275求調劑 +10 | jjjjjjjjjjl 2026-03-27 | 10/500 |
|
|
[考研] 一志愿上海理工能源動力(085800)310分求調劑 +3 | zhangmingc 2026-03-27 | 4/200 |
|
|
[考研] 085600材料與化工調劑 +10 | A-哆啦Z夢 2026-03-23 | 16/800 |
|
|
[考研] 復試調劑,一志愿南農(nóng)083200食品科學與工程 +5 | XQTJZ 2026-03-26 | 5/250 |
|
|
[考研] 一志愿鄭大085600,310分求調劑 +5 | 李瀟可 2026-03-26 | 5/250 |
|
|
[考研] 329求調劑 +7 | 鈕恩雪 2026-03-25 | 7/350 |
|
|
[考研] 【雙一流院校新能源、環(huán)境材料,材料加工與模擬招收大量調劑】 +4 | Higraduate 2026-03-22 | 8/400 |
|
|
[考研] 材料調劑 +3 | iwinso 2026-03-23 | 3/150 |
|
|
[考研] 求調劑 +6 | 研研,接電話 2026-03-24 | 7/350 |
|
|
[考博] 26申博自薦 +3 | whh869393 2026-03-24 | 3/150 |
|
|
[考研] 一志愿重慶大學085700資源與環(huán)境,總分308求調劑 +7 | 墨墨漠 2026-03-23 | 8/400 |
|
|
[考研] 328求調劑 +4 | LHHL66 2026-03-23 | 4/200 |
|