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淺笑包包新蟲 (小有名氣)
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[求助]
assay of ribonuclease activity
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Ten microliters of protein (0.25 mgÆml)1) and 10 ll of saffron leaf total RNA (10 lgÆll)1) were mixed with 100 ll 50 mm MES (pH 6.0) and incubated at 50 C for 30 min. The reaction was stopped by adding 1 ml of ice-cold 4 m LiCl to the mixture, and incubated in ice for 15 min. Insoluble leaf total RNA was removed by centrifugation (10,000 g) for 15 min at 4 C. The supernatant containing soluble leaf total RNA was quantified by UV absorbance at 260 nm in a spectrophotometer (Lan Optics). One unit of enzyme was defined as the amount of protein causing an increase of absorption of 1.0 after 30 min of incubation. The positive and negative controls were RNase A and the heated CsPR10 (100 C for 10 min), respectively. 上述來自文獻(xiàn) 請問是什么意思?為什么要把蛋白質(zhì)與RNA融合?融合后又如何表達(dá)出了它的核糖核苷酸活性? |

新蟲 (小有名氣)

鐵桿木蟲 (職業(yè)作家)
鐵桿木蟲 (職業(yè)作家)
新蟲 (小有名氣)

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