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[求助]
關于2^-△△CT結果的處理
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PCR問題集錦 |
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It is a game of mathematics! OK, let's say, if you had 100 apples at the beginning, then you lost 50, only 50 in your hand. We can say that your apples now only 10% of your original quantities. Or, if you want to say, how many times did you get your decrease of your apples comparing with your original? It should be 2 time decrease (減少一倍 or 原來是現在的二倍,100/50=2). Right? OK, so far so good. Let's put it in your case. For your case, you can use 2^-△△CT directly and put your results there, the last one is 0.1285. But if you want to say in another way (like the apple case above), how many times your sample decrease comparing with your non-treatment, you could not say 0.1285, it display as -(1/x). Here 1/x means the number of times you compare your treatment with your non-treatment. "-" means that is a decrease. 1/0.1285=7.8, so it means your original group is as 7.8 times much as your 48hr treatment samples. It always happens when we want to emphasize the importance of something. If you say after 48 hours, the target decrease to 0.1285 of the control group, it sounds not as that kind of shock effects as the target decrease around 7 times (7.8-1). We have to admit that sometimes (maybe always) how to display your data even more important nowadays. Hope it helpful. 這個是另外一個網站一個老師為我的解答,就是一個數學游戲,我一時沒有轉過來,貼上來大家可以看看,呵呵!謝謝大家! |
鐵桿木蟲 (職業(yè)作家)
金蟲 (正式寫手)

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