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夢(mèng)在農(nóng)大銀蟲 (正式寫手)
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[求助]
急急�。�！這兩個(gè)圖什么意思？
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這兩個(gè)圖的縱坐標(biāo)是啥意思？下面是圖示。這篇文章的題目是： Two Novel GPCR-Type G Proteins Are Abscisic Acid Receptors in Arabidopsis Figure 7. Specific ABA Binding by GTG1 and GTG2 Each experiment was repeated five times and data were averaged. Values are means ± SEM. Except as described in (C), the reaction mix included 5 mM GDP. (A) Both GTG1 and GTG2 show saturable ABA binding with increasing concentrations of 3H-ABA. No binding was detected when BSA instead of GTG1 or GTG2 was used or by using 3H-benzoic acid (BnzA) instead of 3H-ABA. Inset shows Scatchard plot analysis. Parameters derived for GTG1 are KD = 35.8 ± 2.6 nM, with R2 = 0.98 and for GTG2 are KD = 41.2 ± 3.1, with R2 = 0.97. (B) ABA binding by GTG1 and GTG2 is competed by (±)-ABA but not by the biologically inactive stereoisomer, (-)ABA. One hundred nanomolar 3H-ABA was used, with increasing concentrations of cold ABA as indicated. 在論文里的描述是：The G protein activity and GPCR-like topology of GTG proteins along with the significantly ABA hyposensitive phenotypes of gtg1 gtg2 mutants led to the intriguing possibility that the GTG proteins constitute all or part of an unusual class of G protein-coupled ABA perception complex(es). To test this possibility, we carried out direct ABAbinding assays with 3H-ABA and purified recombinant GTG proteins. Both GTG1 and GTG2 proteins showed saturable and specific 3H-ABA binding, with apparent KD (dissociation constant) values of 35.8 nM and 41.2 nM, respectively (Figure 7A). GTG proteins did not bind 3H-benzoic acid, another weak acid used as a negative control. The ABA binding of GTG1 and GTG2 could be effectively competed with unlabeled biologically active (±)-ABA, but not by the biologically inactive ()-ABA isomer (Figure 7B). Following the implication that the GTG proteins do not bind the ()-ABA isomer, the above KD values would then be halved. No 3H-ABA binding was observed when BSA or GPA1 was used as a control for nonspecific binding (Figures 7A and 7C). 求詳細(xì)解答，非常感謝！�。� 圖7.png |
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