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關于血漿蛋白!
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一般納米材料進入人體后,表面往往會吸附一層血漿蛋白,然后有可能導致被巨噬細胞吞噬。我想問的是,納米材料被巨噬細胞所吞噬肯定是由于血漿蛋白的吸附所引起的,不過為何單個或者幾個血漿蛋白的聚合體在人體內不會被巨噬細胞所吞噬呢?或者說,巨噬細胞是如何區(qū)分它吞進的是納米蛋白復合體還是血漿蛋白呢(特別是納米材料尺寸不是很大的情況下)? [ Last edited by dinghuachen on 2013-10-3 at 20:55 ] |
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Here is an excellent paper from the Discher lab to address your question: Science. 2013 Feb 22;339(6122):971-5. doi: 10.1126/science.1229568, Minimal "Self" peptides that inhibit phagocytic clearance and enhance delivery of nanoparticles. Abstract ''Foreign particles and cells are cleared from the body by phagocytes that must also recognize and avoid clearance of "self" cells. The membrane protein CD47 is reportedly a "marker of self" in mice that impedes phagocytosis of self by signaling through the phagocyte receptor CD172a. Minimal "Self" peptides were computationally designed from human CD47 and then synthesized and attached to virus-size particles for intravenous injection into mice that express a CD172a variant compatible with hCD47. Self peptides delay macrophage-mediated clearance of nanoparticles, which promotes persistent circulation that enhances dye and drug delivery to tumors. Self-peptide affinity for CD172a is near the optimum measured for human CD172a variants, and Self peptide also potently inhibits nanoparticle uptake mediated by the contractile cytoskeleton. The reductionist approach reveals the importance of human Self peptides and their utility in enhancing drug delivery and imaging.'' |
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