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轉(zhuǎn)錄因子 已有2人參與
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| 本人并非生物學(xué)出身,對轉(zhuǎn)錄因子不是很了解,現(xiàn)在看文獻(xiàn)時有一個疑惑。作者實(shí)驗(yàn)用轉(zhuǎn)錄組測序方法證實(shí)響應(yīng)細(xì)胞外營養(yǎng)元素A缺乏的若干上調(diào)基因是由轉(zhuǎn)錄因子B調(diào)控的,而A缺乏條件下轉(zhuǎn)錄因子B的基因表達(dá)量沒有發(fā)生改變。請問轉(zhuǎn)錄因子B可能是怎樣調(diào)控這些基因表達(dá)的呢(這個在文獻(xiàn)中并沒有提到過)?謝謝 |
銅蟲 (小有名氣)
銅蟲 (小有名氣)
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hello everyone, i have finished differential expression analysis of my RNA-seq data and retrieve the transcripts of transcription factors(TF) from differentially expressed unigenes and non differentially expressed unigenes. then i have categorized those TFs to different TF families. then i realized there are many TFs sequences which blong to the same TF family overly expressed than some other TF families, some parts of my data: TF_families Differential Unchanged AP-2 0 2 ARID 2 5 bHLH 8 67 CG-1 1 1 COE 0 2 CP2 0 4 CSD 0 4 CSL 0 1 CUT 1 6 DDT 0 1 DM 0 6 E2F 0 3 ETS 2 10 Fork_head 2 22 . . . and i wanna find most expressed TF families in my DE TF sets. i saw a several papers analysed this kind of data and they used fisher's exact test for that. i searched some materials about fisher's exact test, but in those example fisher's test used for 2x2 contingency table, no more. here i still have no idea how to use fisher's test to my data. so could anyone explain to me how could i do solve this and is there any tool i can use for the analysis ? any suggestion would be highly appreciated. |
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