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sc-zsc金蟲 (小有名氣)
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[交流]
【求助】確定控制一顏色性狀的基因型 已有1人參與
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| 現(xiàn)在有兩親本(P1:綠色,P2:黑色,數(shù)量足夠多且為一種基因型),現(xiàn)在要做的是通過正反交、雜交及回交等手段后代的表現(xiàn)型來推測其位置——細胞質(zhì)或細胞核,并不是要精確到DNA分子上的位置。另外,還要能推測是幾對基因控制的——一對、兩對或多對。所以不用到分子方面,就是遺傳方面簡單的問題。 我在這方面就一“菜鳥”,希望能有高手指點一下,或給點類似方面的資料。先行謝過!注:最好的至少10個金幣,其余的視情況給。 |
木蟲 (正式寫手)
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呵呵,這個問題應(yīng)該是一個基礎(chǔ)的遺傳學方面的問題。我對遺傳雖然略懂一二,但是沒有具體的實踐經(jīng)驗,所以在此不說自己的見解。 推薦給你一篇文章吧,是關(guān)于三文魚肉顏色遺傳的。三文魚的顏色有兩種紅色和白色,然后他們通過你提到的方法,確定了有幾個基因位點和幾個等位基因控制的問題。希望對你有所幫助。 When Parameters in Dynamic Models Become Phenotypes: A Case Study on Flesh Pigmentation in the Chinook Salmon (Oncorhynchus tshawytscha) http://www.genetics.org/cgi/content/full/179/2/1113 你可以先看看摘要,如果感興趣可以接著看全文: The Pacific chinook salmon occurs as both white- and red-fleshed populations, with the flesh color type (red or white) seemingly under strong genetic influence. Previously published data on crosses between red- and white-fleshed individuals cannot be reconciled with a simple Mendelian two-locus, two-allele model, pointing to either a more complex inheritance pattern or the existence of gene interactions. Here we show that a standard single-locus, three-allele model can fully explain these data. Moreover, by implementing the single-locus model at the parameter level of a previously developed mathematical model describing carotenoid dynamics in salmon, we show that variation at a single gene involved in the muscle uptake of carotenoids is able to explain the available data. This illustrates how such a combined approach can generate biological understanding that would not be possible in a classical population genetic explanatory structure. An additional asset of this approach is that by allowing parameters to become phenotypes obeying a given genetic model, biological interpretations of mechanisms involved at a resolution level far beyond what is built into the original dynamic model are made possible. These insights can in turn be exploited in experimental studies as well as in construction of more detailed models. [ Last edited by zwdbordeaux on 2009-12-28 at 20:35 ] |

金蟲 (小有名氣)
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