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xujiangli0金蟲 (小有名氣)
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[交流]
【求助/交流】求助幫忙BLAST序列比對(duì)
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本人想要做與我的菌種序列的進(jìn)化樹，但是不會(huì)用BLAST與GenBank中其他序列比對(duì)，請(qǐng)大家?guī)兔Ρ葘?duì)一下，然后把結(jié)果下載給我，或者教我怎么比對(duì)和下載結(jié)果，萬分感謝！急用。。。 本人的序列如下tgcagttgag cgctgaaggt tggtacttgt accgactgga tgagcagcga acgggtgagt aacgcgtggg gaatctgcct ttgagcgggg gacaacattt ggaaacgaat gctaataccg ttaaacacaa gttttaagtt tgaaagatgc aattgcatca ctcaaagatg atcccgcgtt gtattagcta gttggtgagg taaaggctca ccaaggcgat gatacatagccgacctgaga gggtgatcgg ccacattggg actgagacac ggcccaaact cctacgggag gcagcagtag ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt ggtagagaag aacgttggtg agagtggaaa gctcatcaag tgacggtaac tacccagaaa gggacggcta actacgtgcc agcagccgcggtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag cgcaggtggt ttattaagtc tggtgtaaaa ggcagtggct caaccattgt atgcattgga aactggtaga cttgagtgca ggagaggaga gtggaattcc atgtgtagcg gtgaaatgcg tagatatatggaggaacacc ggtggcgaaa gcggctctct ggcctgtaac tgacactgag gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctag tgtagggagc tataagttct ctgtatcgca gctaacgcaa taagcactcc gcctggggagtacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcatgtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatact cgtgctattcctagagatag gaagttcctt cgggacacgg gatacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccctattg ttagttgccatcattaagtt gggcactcta acgagactgc cggtgataaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg tgctacaatg gatggtacaa cgagtcgcga gacagtgatg tttagctaat ctcttaaaac cattctcagt tcggattgta ggctgcaact cgcctacatg aagtcggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtac |
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★ ★ ★
amisking(金幣+3):歡迎交流！ 2010-08-18 21:58:10
amisking(金幣+3):歡迎交流！ 2010-08-18 21:58:10
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你先進(jìn)NCBI網(wǎng)站http://www.ncbi.nlm.nih.gov/，點(diǎn)右邊的BLAST。 在新窗口中的BLAST Assembled RefSeq Genomes。下點(diǎn)擊Microbes 微生物。 在 Enter your query sequence as Accession/GI or FASTA: 下面的框中貼入你的序列。點(diǎn)一下下面的sellect all. 再點(diǎn)下BLAST。 就可以看結(jié)果了。 你的結(jié)果：乳酸菌和鏈球菌也許匹配點(diǎn)。自己看吧。我不是學(xué)微生物的，所以說的很主觀，但基本就是上面的方法（初級(jí)版） 下面分別是：基因號(hào)，物種，匹配分值（越高越匹配），e值（越低越匹配） ref|NC_013656.1| Lactococcus lactis subsp. lactis KF147, comp... 2407 0.0 ref|NC_002662.1| Lactococcus lactis subsp. lactis Il1403, com... 2407 0.0 ref|NC_008527.1| Lactococcus lactis subsp. cremoris SK11, com... 2390 0.0 ref|NC_009004.1| Lactococcus lactis subsp. cremoris MG1363, c... 2357 0.0 ref|NZ_ABJK02000007.1| Streptococcus infantarius subsp. infan... 1807 0.0 ref|NZ_ABJK02000017.1| Streptococcus infantarius subsp. infan... 1801 0.0 ref|NZ_ADVN01000004.1| Streptococcus parasanguinis ATCC 15912... 1792 0.0 ref|NZ_AAJO01000018.1| Streptococcus agalactiae 18RS21 s_agal... 1783 0.0 ref|NZ_AAJS01000005.1| Streptococcus agalactiae H36B s_agalac... 1781 0.0 ref|NZ_AAJS01000007.1| Streptococcus agalactiae H36B s_agalac... 1781 0.0 ref|NZ_AAJS01000028.1| Streptococcus agalactiae H36B s_agalac... 1781 0.0 ref|NZ_AAJR01000001.1| Streptococcus agalactiae COH1 s_agalac... 1781 0.0 ref|NZ_AAJR01000009.1| Streptococcus agalactiae COH1 s_agalac... 1781 0.0 ref|NZ_AAJR01000028.1| Streptococcus agalactiae COH1 s_agalac... 1781 0.0 ref|NZ_AAJQ01000002.1| Streptococcus agalactiae CJB111 s_agal... 1781 0.0 ref|NZ_AAJQ01000013.1| Streptococcus agalactiae CJB111 s_agal... 1781 0.0 ref|NZ_AAJQ01000017.1| Streptococcus agalactiae CJB111 s_agal... 1781 0.0 ref|NZ_AAJQ01000027.1| Streptococcus agalactiae CJB111 s_agal... 1781 0.0 ref|NZ_AAJQ01000049.1| Streptococcus agalactiae CJB111 s_agal... 1781 0.0 ref|NZ_AAJQ01000070.1| Streptococcus agalactiae CJB111 s_agal... 1781 0.0 ref|NZ_AAJP01000024.1| Streptococcus agalactiae 515 s_agalact... 1781 0.0 ref|NC_007432.1| Streptococcus agalactiae A909, complete genome 1781 0.0 ref|NC_004368.1| Streptococcus agalactiae NEM316, complete ge... 1781 0.0 ref|NC_004116.1| Streptococcus agalactiae 2603V/R, complete g... 1781 0.0 ref|NZ_AAJO01000124.1| Streptococcus agalactiae 18RS21 s_agal... 1773 0.0 ref|NC_012891.1| Streptococcus dysgalactiae subsp. equisimili... 1773 0.0 ref|NZ_AAJO01000006.1| Streptococcus agalactiae 18RS21 s_agal... 1770 0.0 ref|NC_013798.1| Streptococcus gallolyticus UCN34, complete g... 1768 0.0 ref|NZ_AAJO01000021.1| Streptococcus agalactiae 18RS21 s_agal... 1760 0.0 ref|NZ_ADMV01000001.1| Streptococcus oralis ATCC 35037 contig... 1759 0.0 ref|NZ_AAJO01000130.1| Streptococcus agalactiae 18RS21 s_agal... 1759 0.0 ref|NZ_AAJO01000148.1| Streptococcus agalactiae 18RS21 s_agal... 1757 0.0 ref|NZ_AAJO01000012.1| Streptococcus agalactiae 18RS21 s_agal... 1746 0.0 ref|NZ_AAFV01000024.1| Streptococcus pyogenes M49 591 SpyoM01... 1746 0.0 gb|ACRK01000025.1| Streptococcus sp. M143 cont1.25, whole gen... 1744 0.0 ref|NC_011375.1| Streptococcus pyogenes NZ131 chromosome, com... 1740 0.0 ref|NC_009332.1| Streptococcus pyogenes str. Manfredo, comple... 1740 0.0 ref|NC_008024.1| Streptococcus pyogenes MGAS10750 chromosome,... 1740 0.0 ref|NC_008023.1| Streptococcus pyogenes MGAS2096 chromosome, ... 1740 0.0 ref|NC_008022.1| Streptococcus pyogenes MGAS10270 chromosome,... 1740 0.0 ref|NC_008021.1| Streptococcus pyogenes MGAS9429 chromosome, ... 1740 0.0 ref|NC_007297.1| Streptococcus pyogenes MGAS5005 chromosome, ... 1740 0.0 ref|NC_007296.1| Streptococcus pyogenes MGAS6180 chromosome, ... 1740 0.0 ref|NC_006086.1| Streptococcus pyogenes MGAS10394 chromosome,... 1740 0.0 ref|NC_004606.1| Streptococcus pyogenes SSI-1, complete genome 1740 0.0 ref|NC_004070.1| Streptococcus pyogenes MGAS315, complete genome 1740 0.0 ref|NC_002737.1| Streptococcus pyogenes M1 GAS chromosome, co... 1740 0.0 ref|NC_013853.1| Streptococcus mitis B6, complete genome 1738 0.0 ref|NC_006449.1| Streptococcus thermophilus CNRZ1066 chromoso... 1736 0.0 ref|NC_006448.1| Streptococcus thermophilus LMG 18311 chromos... 1736 0.0 ref|NC_003485.1| Streptococcus pyogenes MGAS8232, complete ge... 1735 0.0 ref|NZ_ACLO01000035.1| Streptococcus salivarius SK126 contig0... 1731 0.0 ref|NC_009009.1| Streptococcus sanguinis SK36, complete genome 1731 0.0 ref|NC_008532.1| Streptococcus thermophilus LMD-9, complete g... 1731 0.0 emb|CACE01000039.1| Streptococcus pneumoniae SPN1041 whole ge... 1727 0.0 ref|NZ_ABGF01000013.1| Streptococcus pneumoniae CDC0288-04 gc... 1727 0.0 ref|NC_012467.1| Streptococcus pneumoniae P1031, complete genome 1727 0.0 ref|NC_011900.1| Streptococcus pneumoniae ATCC 700669, comple... 1727 0.0 ref|NC_011134.1| Streptococcus equi subsp. zooepidemicus MGCS... 1725 0.0 gb|ABWC01000018.1| Streptococcus pneumoniae BS397 ctg138, who... 1722 0.0 gb|ABWB01000066.1| Streptococcus pneumoniae BS457 ctg00014, w... 1722 0.0 gb|ABWA01000073.1| Streptococcus pneumoniae BS458 ctg00092, w... 1722 0.0 gb|ABWU01000026.1| Streptococcus pneumoniae SP-BS293 ctg169, ... 1722 0.0 gb|ABWQ01000022.1| Streptococcus pneumoniae SP14-BS292 ctg155... 1722 0.0 gb|ADHN01000066.1| Streptococcus pneumoniae BS455 ctg00069, w... 1722 0.0 emb|CACJ01000026.1| Streptococcus pneumoniae SPN061370 whole ... 1722 0.0 emb|CACI01000019.1| Streptococcus pneumoniae SPN072838 whole ... 1722 0.0 emb|CACH01000022.1| Streptococcus pneumoniae SPN021198 whole ... 1722 0.0 ref|NZ_ABGH01000001.1| Streptococcus pneumoniae MLV-016 gcont... 1722 0.0 ref|NZ_ABGH01000004.1| Streptococcus pneumoniae MLV-016 gcont... 1722 0.0 ref|NZ_ABGH01000008.1| Streptococcus pneumoniae MLV-016 gcont... 1722 0.0 ref|NZ_ABGH01000011.1| Streptococcus pneumoniae MLV-016 gcont... 1722 0.0 ref|NZ_ABGH01000012.1| Streptococcus pneumoniae MLV-016 gcont... 1722 0.0 ref|NZ_ABGH01000014.1| Streptococcus pneumoniae MLV-016 gcont... 1722 0.0 ref|NZ_ABGH01000022.1| Streptococcus pneumoniae MLV-016 gcont... 1722 0.0 ref|NZ_ABGF01000005.1| Streptococcus pneumoniae CDC0288-04 gc... 1722 0.0 ref|NZ_ABGF01000006.1| Streptococcus pneumoniae CDC0288-04 gc... 1722 0.0 ref|NZ_ABGF01000008.1| Streptococcus pneumoniae CDC0288-04 gc... 1722 0.0 ref|NZ_ABGF01000011.1| Streptococcus pneumoniae CDC0288-04 gc... 1722 0.0 ref|NZ_ABGF01000016.1| Streptococcus pneumoniae CDC0288-04 gc... 1722 0.0 ref|NZ_ABFT01000010.1| Streptococcus pneumoniae CDC1087-00 gc... 1722 0.0 ref|NZ_ABFT01000018.1| Streptococcus pneumoniae CDC1087-00 gc... 1722 0.0 ref|NZ_ABFT01000050.1| Streptococcus pneumoniae CDC1087-00 gc... 1722 0.0 ref|NZ_ABAG01000013.1| Streptococcus pneumoniae SP23-BS72 ctg... 1722 0.0 ref|NZ_AAZZ01000003.1| Streptococcus pneumoniae SP3-BS71 ctg5... 1722 0.0 ref|NZ_ABAE01000014.1| Streptococcus pneumoniae SP18-BS74 ctg... 1722 0.0 ref|NZ_ABAD01000031.1| Streptococcus pneumoniae SP14-BS69 ctg... 1722 0.0 ref|NZ_AAGY02000123.1| Streptococcus pneumoniae TIGR4 SpneT_0... 1722 0.0 ref|NC_012466.1| Streptococcus pneumoniae JJA, complete genome 1722 0.0 ref|NC_011072.1| Streptococcus pneumoniae G54 chromosome, com... 1722 0.0 ref|NC_003028.3| Streptococcus pneumoniae TIGR4 chromosome, c... 1722 0.0 ref|NC_010582.1| Streptococcus pneumoniae CGSP14, complete ge... 1722 0.0 ref|NC_010380.1| Streptococcus pneumoniae Hungary19A-6, compl... 1722 0.0 ref|NZ_ABFS01000003.1| Streptococcus pneumoniae CDC1873-00 gc... 1720 0.0 ref|NZ_ABFS01000009.1| Streptococcus pneumoniae CDC1873-00 gc... 1720 0.0 ref|NZ_ABFS01000019.1| Streptococcus pneumoniae CDC1873-00 gc... 1720 0.0 ref|NZ_ABFS01000026.1| Streptococcus pneumoniae CDC1873-00 gc... 1720 0.0 ref|NZ_ABFS01000034.1| Streptococcus pneumoniae CDC1873-00 gc... 1720 0.0 ref|NC_012470.1| Streptococcus equi subsp. zooepidemicus, com... 1718 0.0 ref|NC_012471.1| Streptococcus equi subsp. equi 4047, complet... 1718 0.0 [ Last edited by adslye on 2010-8-18 at 21:23 ] |
2樓2010-08-18 21:21:42
xp198766
鐵桿木蟲 (著名寫手)
小木蟲職業(yè)打醬油滴~~！
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★ ★ ★
amisking(金幣+3):good！ 2010-08-18 21:58:22
xujiangli0(金幣+10):能不能具體教我怎么下載這個(gè)序列結(jié)果的，謝謝啊 2010-08-19 16:19:38
amisking(金幣+3):good！ 2010-08-18 21:58:22
xujiangli0(金幣+10):能不能具體教我怎么下載這個(gè)序列結(jié)果的，謝謝啊 2010-08-19 16:19:38
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給你一個(gè)有99個(gè)序列的結(jié)果，都是BLAST前面的99個(gè)，有一個(gè)是基因組的，沒有給你 http://g.zhubajie.com/urllink.php?id=9718642ylhzlgq3q1orlx95 |
3樓2010-08-18 21:22:24
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