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liying841216金蟲 (初入文壇)
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[求助]
300金幣求助翻譯小碩論文摘要
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| 背景與目的：實驗通過建立模擬人皮膚取皮區(qū)創(chuàng)面來檢測生長因子TGF-β1和VEGF在創(chuàng)面愈合以及瘢痕增生方面的作用，為臨床應(yīng)用新型藻酸鹽敷料治療取皮區(qū)創(chuàng)面的應(yīng)用提供數(shù)據(jù)依據(jù)，方法：主要將藻酸鹽敷料應(yīng)用于版納微型豬近交系中厚皮取皮區(qū)創(chuàng)面，通過檢測不同時間點（取皮后0、3、7、14、28天）創(chuàng)面愈合過程中及創(chuàng)面愈合后的生長因子TGF-β1、VEGF的mRNA表達(dá)情況，探討新型藻酸鹽敷料對創(chuàng)面愈合的生物學(xué)機(jī)制及減輕瘢痕增生的具體作用機(jī)制。結(jié)果：構(gòu)建的取皮區(qū)創(chuàng)面動物模型，從大體外觀上觀測，其藻酸鹽治療組與凡士林對照組存在明顯差異，治療組創(chuàng)面愈合時間少于對照組(P<0.05),具有統(tǒng)計學(xué)差異。取皮區(qū)創(chuàng)面愈合過程中不同時間點的治療組與對照組的基因表達(dá)情況表明，不同的時間點之間有顯著差異（P＜0.01），治療與否有顯著性差異（P＝0.025），不同豬之間無統(tǒng)計學(xué)差異。TGF-β1在0、3、7、14、28天的表達(dá)量為：治療組-5.068±0.286，-4.131±0.396，-3.294±0.516，-3.514±0.374，-4.695±0.809；對照組-5.068±0.286，-4,457±0.450，-4.262±0.512，-4.025±0.365，-4.485±0.233。TGF-β1表現(xiàn)為隨著創(chuàng)面的愈合其表達(dá)上升，至中期表達(dá)升至最高，之后表達(dá)下降，在創(chuàng)面完全愈合后其表達(dá)量趨于正常水平。其中在14天藻酸鹽治療組與凡士林對照組之間TGF-β1表達(dá)有顯著差異。VEGF的mRNA表達(dá)量的統(tǒng)計結(jié)果與TGF-β1的結(jié)果相似，其中7天藻酸鹽治療組與凡士林對照組之間VEGF的mRNA的表達(dá)有顯著差異。VEGF在0、3、7、14、28天的表達(dá)量為：治療組-3.461±0.279,-2.726±0.425,-1.722±0.097,-2.401±0.341,-2.902±0.437,對照組為-2.928±0.385，-2.522±0.319，-2.877±0.517，-3.230±0.451。結(jié)論：通過構(gòu)建取皮后中厚皮創(chuàng)面動物模型，成功構(gòu)建了檢測取皮區(qū)創(chuàng)面組織中VEGF、TGF-β1的mRNA的SYBR GreenⅠ實時熒光定量檢測方法，揭示藻酸鹽敷料對促進(jìn)創(chuàng)面愈合有重要作用，且主要與調(diào)控細(xì)胞因子的表達(dá)相關(guān)，為解決創(chuàng)面愈合減少瘢痕增生等臨床棘手問題提供了可靠的理論依據(jù)。 |
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jfly
捐助貴賓 (正式寫手)
驚天動地大好人
- 翻譯EPI: 1
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- 金幣: 1743.6
- 帖子: 333
- 在線: 10.6小時
- 蟲號: 37737
- 注冊: 2004-02-16
- 性別: GG
- 專業(yè): 教學(xué)論
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
sltmac(金幣+2): 歡迎常來本版交流~~ 2011-04-24 18:00:27
ringzhu(金幣+50): 歡迎常來~~ 2011-04-28 18:45:55
sltmac(金幣+2): 歡迎常來本版交流~~ 2011-04-24 18:00:27
ringzhu(金幣+50): 歡迎常來~~ 2011-04-28 18:45:55
|
樓主應(yīng)該自己翻譯好了讓人修改。 樓上翻譯問題比較多（太多we、時態(tài)不對等等），我一點點改改試試看。專業(yè)術(shù)語就無能為力了。 Background and Objective: The impact of growth factors TGF-β1 and VEGF on wound healing and scar formation was detected through the establishment of simulated human skin in access site wound. The data generated in this study can be used in clinical application of new alginate dressings on skin treatment Wounds. Method: Alginate dressing was used in inbred Banna minipig check site wounds in the thick. The mRNA expressions of growth factors TGF-β1 and VEGF were detected in and after the process of wound healing (more specifically, 0, 3, 7, 14, and 28 days after taking the skin). The collected data were then applied to explore the biological mechanisms of new alginate dressing in wound healing and scar reducing. 待續(xù)吧…… |
6樓2011-04-24 02:42:23
zhenghuadong
木蟲 (著名寫手)
勤奮的蟲子
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2樓2011-04-20 18:07:34
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
ringzhu(金幣+2): 歡迎常來翻譯版~~ 2011-04-21 20:27:38
ringzhu(金幣+50, 翻譯EPI+1): 歡迎常來~~ 2011-04-28 18:46:48
ringzhu(金幣+50): 2011-04-28 18:46:55
ringzhu(金幣+50): 2011-04-28 18:47:00
ringzhu(金幣+2): 歡迎常來翻譯版~~ 2011-04-21 20:27:38
ringzhu(金幣+50, 翻譯EPI+1): 歡迎常來~~ 2011-04-28 18:46:48
ringzhu(金幣+50): 2011-04-28 18:46:55
ringzhu(金幣+50): 2011-04-28 18:47:00
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Background and Objective：we detect the influence of growth factor TGF-β1 and VEGF in wound healing and scar formation through the establishment of simulation of people skin in access site wound. it provides the data for clinical application of new alginate dressings on skin treatment Wounds . Method:we mainly applied alginate dressing in inbred Banna minipig check site wounds in the thick. we detect the different time points (0,3,7,14,28 days after taking the skin) and wound healing process of wound healing growth factor TGF-β1, VEGF expression of the mRNA to explore new alginate dressing on wound healing biological mechanisms and the specific role of reducing scar formation mechanism. 吃完中飯回來繼續(xù)翻  |
3樓2011-04-21 11:14:49
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
ringzhu(金幣+50): 2011-04-28 18:46:28
ringzhu(金幣+50): 2011-04-28 18:46:28
| Results: we observe from the general appearance of construction of access site wound model, , the Vaseline alginate treatment group and control group was significantly different, healing time of the treatment group was shorter than the control group (P <0.05), which is significantly different. Skin graft taken at different time points during the treatment and control groups showed that the gene expression of the different time points were significantly different(P <0.01), whether the treatment significantly different or not (P = 0.025), no statistical difference between the different pigs exists. |
4樓2011-04-21 11:22:13
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