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liying841216金蟲(chóng) (初入文壇)
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[求助]
300金幣求助翻譯小碩論文摘要
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| 背景與目的：實(shí)驗(yàn)通過(guò)建立模擬人皮膚取皮區(qū)創(chuàng)面來(lái)檢測(cè)生長(zhǎng)因子TGF-β1和VEGF在創(chuàng)面愈合以及瘢痕增生方面的作用，為臨床應(yīng)用新型藻酸鹽敷料治療取皮區(qū)創(chuàng)面的應(yīng)用提供數(shù)據(jù)依據(jù)，方法：主要將藻酸鹽敷料應(yīng)用于版納微型豬近交系中厚皮取皮區(qū)創(chuàng)面，通過(guò)檢測(cè)不同時(shí)間點(diǎn)（取皮后0、3、7、14、28天）創(chuàng)面愈合過(guò)程中及創(chuàng)面愈合后的生長(zhǎng)因子TGF-β1、VEGF的mRNA表達(dá)情況，探討新型藻酸鹽敷料對(duì)創(chuàng)面愈合的生物學(xué)機(jī)制及減輕瘢痕增生的具體作用機(jī)制。結(jié)果：構(gòu)建的取皮區(qū)創(chuàng)面動(dòng)物模型，從大體外觀上觀測(cè)，其藻酸鹽治療組與凡士林對(duì)照組存在明顯差異，治療組創(chuàng)面愈合時(shí)間少于對(duì)照組(P<0.05),具有統(tǒng)計(jì)學(xué)差異。取皮區(qū)創(chuàng)面愈合過(guò)程中不同時(shí)間點(diǎn)的治療組與對(duì)照組的基因表達(dá)情況表明，不同的時(shí)間點(diǎn)之間有顯著差異（P＜0.01），治療與否有顯著性差異（P＝0.025），不同豬之間無(wú)統(tǒng)計(jì)學(xué)差異。TGF-β1在0、3、7、14、28天的表達(dá)量為：治療組-5.068±0.286，-4.131±0.396，-3.294±0.516，-3.514±0.374，-4.695±0.809；對(duì)照組-5.068±0.286，-4,457±0.450，-4.262±0.512，-4.025±0.365，-4.485±0.233。TGF-β1表現(xiàn)為隨著創(chuàng)面的愈合其表達(dá)上升，至中期表達(dá)升至最高，之后表達(dá)下降，在創(chuàng)面完全愈合后其表達(dá)量趨于正常水平。其中在14天藻酸鹽治療組與凡士林對(duì)照組之間TGF-β1表達(dá)有顯著差異。VEGF的mRNA表達(dá)量的統(tǒng)計(jì)結(jié)果與TGF-β1的結(jié)果相似，其中7天藻酸鹽治療組與凡士林對(duì)照組之間VEGF的mRNA的表達(dá)有顯著差異。VEGF在0、3、7、14、28天的表達(dá)量為：治療組-3.461±0.279,-2.726±0.425,-1.722±0.097,-2.401±0.341,-2.902±0.437,對(duì)照組為-2.928±0.385，-2.522±0.319，-2.877±0.517，-3.230±0.451。結(jié)論：通過(guò)構(gòu)建取皮后中厚皮創(chuàng)面動(dòng)物模型，成功構(gòu)建了檢測(cè)取皮區(qū)創(chuàng)面組織中VEGF、TGF-β1的mRNA的SYBR GreenⅠ實(shí)時(shí)熒光定量檢測(cè)方法，揭示藻酸鹽敷料對(duì)促進(jìn)創(chuàng)面愈合有重要作用，且主要與調(diào)控細(xì)胞因子的表達(dá)相關(guān)，為解決創(chuàng)面愈合減少瘢痕增生等臨床棘手問(wèn)題提供了可靠的理論依據(jù)。 |
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zhenghuadong
木蟲(chóng) (著名寫(xiě)手)
勤奮的蟲(chóng)子
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- 專(zhuān)業(yè): 光學(xué)信息獲取與處理
2樓2011-04-20 18:07:34
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
ringzhu(金幣+2): 歡迎常來(lái)翻譯版~~ 2011-04-21 20:27:38
ringzhu(金幣+50, 翻譯EPI+1): 歡迎常來(lái)~~ 2011-04-28 18:46:48
ringzhu(金幣+50): 2011-04-28 18:46:55
ringzhu(金幣+50): 2011-04-28 18:47:00
ringzhu(金幣+2): 歡迎常來(lái)翻譯版~~ 2011-04-21 20:27:38
ringzhu(金幣+50, 翻譯EPI+1): 歡迎常來(lái)~~ 2011-04-28 18:46:48
ringzhu(金幣+50): 2011-04-28 18:46:55
ringzhu(金幣+50): 2011-04-28 18:47:00
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Background and Objective：we detect the influence of growth factor TGF-β1 and VEGF in wound healing and scar formation through the establishment of simulation of people skin in access site wound. it provides the data for clinical application of new alginate dressings on skin treatment Wounds . Method:we mainly applied alginate dressing in inbred Banna minipig check site wounds in the thick. we detect the different time points (0,3,7,14,28 days after taking the skin) and wound healing process of wound healing growth factor TGF-β1, VEGF expression of the mRNA to explore new alginate dressing on wound healing biological mechanisms and the specific role of reducing scar formation mechanism. 吃完中飯回來(lái)繼續(xù)翻  |
3樓2011-04-21 11:14:49
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
ringzhu(金幣+50): 2011-04-28 18:46:28
ringzhu(金幣+50): 2011-04-28 18:46:28
| Results: we observe from the general appearance of construction of access site wound model, , the Vaseline alginate treatment group and control group was significantly different, healing time of the treatment group was shorter than the control group (P <0.05), which is significantly different. Skin graft taken at different time points during the treatment and control groups showed that the gene expression of the different time points were significantly different(P <0.01), whether the treatment significantly different or not (P = 0.025), no statistical difference between the different pigs exists. |
4樓2011-04-21 11:22:13
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
ringzhu(金幣+50): 2011-04-28 18:46:21
ringzhu(金幣+50): 2011-04-28 18:46:21
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TGF-β1 expression in 0,3,7,14,28 days as follows:Treatment group, -5.068 ± 0.286, -4.131 ± 0.396, -3.294 ± 0.516, -3.514 ± 0.374, -4.695 ± 0.809; control group, -5.068 ± 0.286, -4,457 ± 0.450, -4.262 ± 0.512, -4.025 ± 0.365, - 4.485 ± 0.233.As TGF-β1 showed increased the expression of wound healing, increased expression of the highest to medium term, after which expression decreased in the wound healed completely after its expression tends to the normal level.Alginate treatment group and control group Vaseline expression of TGF-β1 were significantly different in 14 days.The amount of VEGF mRNA expression was similar to TGF-β1 , however,expression of VEGF mRNA in alginate treatment group and control group Vaseline were significantly different.VEGF expression in 0,3,7,14,28 days as follows: the treatment group -3.461 ± 0.279, -2.726 ± 0.425, -1.722 ± 0.097, -2.401 ± 0.341, -2.902 ± 0.437, control group was -2.928 ± 0.385, -2.522 ± 0.319, -2.877 ± 0.517, -3.230 ± 0.451.Conclusion: After the building to take in the thick skin wound model ,we successfully constructed the SYBR Green Ⅰ quantitative real-time fluorescence detection method which applied to mRNA of test taking site wound tissue VEGF, TGF-β1 , reveals the alginate dressings importangt role in promoting wound healing , and mainly related to regulation of the expression of cytokines.It provides a reliable theoretical basis to address the reduction of scarring wound healing clinical thorny issues. (結(jié)束了) |
5樓2011-04-21 14:20:00
jfly
捐助貴賓 (正式寫(xiě)手)
驚天動(dòng)地大好人
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
sltmac(金幣+2): 歡迎常來(lái)本版交流~~ 2011-04-24 18:00:27
ringzhu(金幣+50): 歡迎常來(lái)~~ 2011-04-28 18:45:55
sltmac(金幣+2): 歡迎常來(lái)本版交流~~ 2011-04-24 18:00:27
ringzhu(金幣+50): 歡迎常來(lái)~~ 2011-04-28 18:45:55
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樓主應(yīng)該自己翻譯好了讓人修改。 樓上翻譯問(wèn)題比較多（太多we、時(shí)態(tài)不對(duì)等等），我一點(diǎn)點(diǎn)改改試試看。專(zhuān)業(yè)術(shù)語(yǔ)就無(wú)能為力了。 Background and Objective: The impact of growth factors TGF-β1 and VEGF on wound healing and scar formation was detected through the establishment of simulated human skin in access site wound. The data generated in this study can be used in clinical application of new alginate dressings on skin treatment Wounds. Method: Alginate dressing was used in inbred Banna minipig check site wounds in the thick. The mRNA expressions of growth factors TGF-β1 and VEGF were detected in and after the process of wound healing (more specifically, 0, 3, 7, 14, and 28 days after taking the skin). The collected data were then applied to explore the biological mechanisms of new alginate dressing in wound healing and scar reducing. 待續(xù)吧…… |
6樓2011-04-24 02:42:23
sll6658110
銅蟲(chóng) (初入文壇)
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7樓2011-04-25 18:03:00
sll6658110
銅蟲(chóng) (初入文壇)
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- 專(zhuān)業(yè): 有機(jī)合成
【答案】應(yīng)助回帖
★ ★ ★ ★ ★
ringzhu(金幣-5): 本版嚴(yán)禁機(jī)器翻譯！！應(yīng)助前請(qǐng)仔細(xì)閱讀本版版規(guī)~~多謝合作 2011-04-28 18:44:31
ringzhu(金幣-5): 本版嚴(yán)禁機(jī)器翻譯！！應(yīng)助前請(qǐng)仔細(xì)閱讀本版版規(guī)~~多謝合作 2011-04-28 18:44:31
| Background and purpose: experiments to simulate the skin by establishing take skin area to detect growth factor wound TGF - beta 1 and VEGF in wound healing and the role of scar tissue hyperplasia for clinical application, new alginate dressings treatment take skin area wound applied to provide data basis, methods: main will alginate dressing applied in miniature pig with coefficient of thick skin take skin area through testing the tissue, at different time points (0, 3 after take skin, 7, 14 and 28 days) coagulant process and the growth factor coagulant TGF - beta 1, VEGF, discusses the mRNA expression of new alginate dressing coagulant biological mechanism and reduce the specific mechanism scar hyperplasia. Results: the construction of animal model take skin area wound from the gross appearance on alginate observation, the treatment group and control group vaseline, there exists significant difference in treatment group wound healing time less than in control group (P < 0.05), has a statistically significant. Take skin area coagulant at different time points in the process of the treatment group and control group, gene expression in different time points that there was a significant difference between (P < 0.01), treatment or not have significant difference (P = 0.025), between different pig was not statistically significant. TGF - beta 1 at 0, 3, 7, 14 and 28 days of express quantity is: treatment group - 5.068 + 4.131 + 0.396 0.286, - 0.516, 3.294 +, - 0.374 3.514 +, - - 4.695 + 0.809; 5.068 + 0.286 group - 0.450 4,457 +, - 0.512, 4.262 +, - 0.365 4.025 +, - - 4.485 + 0.233. TGF - beta 1 performance as the healing of the wound up its expression, to medium express to its hi |
8樓2011-04-25 18:04:40
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