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[求助]
菜鳥(niǎo)問(wèn)題:細(xì)胞保存在液氮罐液相還是氣相中?
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我們實(shí)驗(yàn)室剛開(kāi)始做培養(yǎng)細(xì)胞的實(shí)驗(yàn)。我們以前都是保存在-76度的冰箱里面,這樣可行嗎?現(xiàn)在我們買(mǎi)了一個(gè)Thermo 的CY50900液氮罐,在說(shuō)明書(shū)中是這樣說(shuō)的: Storing Samples in Vapor Phase 1. Remove the 2 bottom-most cryoboxes from each rack. 2. Measure the height of 2 cryoboxes stacked on top of each other. 3. Fill vessel with liquid nitrogen to the height obtained from step 2 using a yardstick to measure liquid level in vessel. 4. Place racks into vessel without the 2 bottom-most cryoboxes. — Samples are now above the liquid nitrogen and are stored in the vapor phase.— Important—A level monitor should always be used when you store your samples in the vapor phase because of the already low levels of liquid nitrogen. Please refer to page 10 for the correct level monitor for your system. 也就是說(shuō)細(xì)胞要儲(chǔ)存在氣態(tài)中,但是我看網(wǎng)上說(shuō)的培養(yǎng)技術(shù)細(xì)胞都是凍存在液相里的。那么到底應(yīng)該把細(xì)胞放在哪里呢? |
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阿郎哥

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