| 5 | 1/1 | 返回列表 |
| 查看: 3466 | 回復(fù): 5 | ||
| 本帖產(chǎn)生 1 個(gè) BioEPI ,點(diǎn)擊這里進(jìn)行查看 | ||
| 當(dāng)前只顯示滿足指定條件的回帖,點(diǎn)擊這里查看本話題的所有回帖 | ||
[求助]
菜鳥(niǎo)問(wèn)題:細(xì)胞保存在液氮罐液相還是氣相中?
|
||
|
我們實(shí)驗(yàn)室剛開(kāi)始做培養(yǎng)細(xì)胞的實(shí)驗(yàn)。我們以前都是保存在-76度的冰箱里面,這樣可行嗎?現(xiàn)在我們買(mǎi)了一個(gè)Thermo 的CY50900液氮罐,在說(shuō)明書(shū)中是這樣說(shuō)的: Storing Samples in Vapor Phase 1. Remove the 2 bottom-most cryoboxes from each rack. 2. Measure the height of 2 cryoboxes stacked on top of each other. 3. Fill vessel with liquid nitrogen to the height obtained from step 2 using a yardstick to measure liquid level in vessel. 4. Place racks into vessel without the 2 bottom-most cryoboxes. — Samples are now above the liquid nitrogen and are stored in the vapor phase.— Important—A level monitor should always be used when you store your samples in the vapor phase because of the already low levels of liquid nitrogen. Please refer to page 10 for the correct level monitor for your system. 也就是說(shuō)細(xì)胞要儲(chǔ)存在氣態(tài)中,但是我看網(wǎng)上說(shuō)的培養(yǎng)技術(shù)細(xì)胞都是凍存在液相里的。那么到底應(yīng)該把細(xì)胞放在哪里呢? |
銅蟲(chóng) (初入文壇)

金蟲(chóng) (小有名氣)
金蟲(chóng) (正式寫(xiě)手)
木蟲(chóng) (小有名氣)
阿郎哥

| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 349求調(diào)劑 +6 | 李木子啊哈哈 2026-03-25 | 6/300 |
|
|---|---|---|---|---|
|
[考研] 求收留 +5 | 1943443204 2026-03-28 | 5/250 |
|
|
[考研] 317分 一志愿南理工材料工程 本科湖工大 求調(diào)劑 +9 | 芋泥小鈴鐺 2026-03-28 | 9/450 |
|
|
[考研] 311求調(diào)劑 +5 | 冬十三 2026-03-24 | 5/250 |
|
|
[考研] 一志愿華北電力大學(xué)能動(dòng)專(zhuān)碩,293,求調(diào)劑 +3 | 15537177284 2026-03-23 | 5/250 |
|
|
[考研] 求調(diào)劑 +3 | QiMing7 2026-03-25 | 4/200 |
|
|
[考研] 317求調(diào)劑 +6 | 十閑wx 2026-03-24 | 6/300 |
|
|
[考研] 299求調(diào)劑 +7 | 嗯嗯嗯嗯2 2026-03-27 | 7/350 |
|
|
[考研] 283求調(diào)劑 +7 | A child 2026-03-28 | 7/350 |
|
|
[考研] 一志愿南昌大學(xué)324求調(diào)劑 +7 | hanamiko 2026-03-27 | 7/350 |
|
|
[考研] 081200-314 +3 | LILIQQ 2026-03-27 | 4/200 |
|
|
[考研] 311求調(diào)劑 +3 | 希望上岸阿小楊 2026-03-23 | 3/150 |
|
|
[考研] 308求調(diào)劑 +7 | 墨墨漠 2026-03-27 | 7/350 |
|
|
[考研] 化學(xué)308分求調(diào)劑 +8 | 你好明天你好 2026-03-23 | 9/450 |
|
|
[考研] 315調(diào)劑 +4 | 0860求調(diào)劑 2026-03-26 | 5/250 |
|
|
[考研] 321求調(diào)劑 +6 | wasdssaa 2026-03-26 | 6/300 |
|
|
[考研] 325求調(diào)劑 +3 | Aoyijiang 2026-03-23 | 3/150 |
|
|
[考研] 081200-11408-276學(xué)碩求調(diào)劑 +3 | 崔wj 2026-03-26 | 3/150 |
|
|
[考研] 302求調(diào)劑 +4 | 錦衣衛(wèi)藤椒 2026-03-25 | 4/200 |
|
|
[考研] 一志愿武理085500機(jī)械專(zhuān)業(yè)總分300求調(diào)劑 +3 | an10101 2026-03-24 | 7/350 |
|