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[求助]
關(guān)于淀粉葡糖糖苷酶AMYLOGLUCOSIDASE
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在sigma買的,是個淀粉葡糖糖苷酶,有同學告訴我為外切酶,作用于1,4,1,6葡萄糖糖苷鍵,但是我具體查不到它的相關(guān)性質(zhì)描述。 求助:它到底是內(nèi)切酶還是外切酶?主要作用的糖苷鍵到底是啥? |

新蟲 (正式寫手)
木蟲 (正式寫手)
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http://www.brenda-enzymes.info/ 在上面的網(wǎng)址中查詢一下吧,應該是兩種酶的混合物可完全水解淀粉生成葡萄糖。 |
鐵桿木蟲 (正式寫手)
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Amyloglucosidase (EC 3.2.1.3):Accepted name: glucan 1,4-α-glucosidase Reaction: Hydrolysis of terminal (1→4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose Other name(s): glucoamylase; amyloglucosidase; γ-amylase; lysosomal α-glucosidase; acid maltase; exo-1,4-α-glucosidase; glucose amylase; γ-1,4-glucan glucohydrolase; acid maltase; 1,4-α-D-glucan glucohydrolase Systematic name: 4-α-D-glucan glucohydrolase Comments: Most forms of the enzyme can rapidly hydrolyse 1,6-α-D-glucosidic bonds when the next bond in the sequence is 1,4, and some preparations of this enzyme hydrolyse 1,6- and 1,3-α-D-glucosidic bonds in other polysaccharides. This entry covers all such enzymes acting on polysaccharides more rapidly than on oligosaccharides. EC 3.2.1.20 α-glucosidase, from mammalian intestine, can catalyse similar reactions. http://www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/3.html |
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