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jiangbo9090木蟲 (小有名氣)
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[求助]
摘要翻譯
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纖維原料是地球上現(xiàn)今最豐富、可再生的廉價的重要資源，對它的應(yīng)用的研究一直是科學界的重點和熱點。纖維素酶是一種高活性生物催化劑。隨著現(xiàn)代微生物技術(shù)的發(fā)展，纖維素酶在飼料、酒精、紡織和食品等領(lǐng)域顯現(xiàn)出巨大的市場潛力。 實驗室課題組對雙功能纖維素酶CbCel9A1CBM各模塊功能研究后發(fā)現(xiàn)其各截短蛋白間存在漸進性和協(xié)同性。為進一步具體從結(jié)構(gòu)-功能的關(guān)系了解各截短蛋白，我們希望能夠?qū)bCel 9A-L和L-CBM3C模塊蛋白經(jīng)行晶體結(jié)構(gòu)的解析，從而能從根本上了解其作用機理。首先，我們對目標蛋白經(jīng)行了Ni-柱親和分離純化，并通過分子篩層析純化發(fā)現(xiàn)純化效果很好。之后根據(jù)SDS-PAGE凝膠電泳對目標蛋白的純度進行了考證后用考馬斯亮藍法對目標蛋白的濃度進行了測定，并同時濃縮蛋白濃度。之后用懸滴式蒸汽擴散法進行大量實驗后，確定了目標蛋白的最優(yōu)結(jié)晶條件并進行了單晶培養(yǎng)。最后集中送往上海光源中國科學院上海應(yīng)用物理研究所進行X-光同步輻射衍射解析晶體結(jié)構(gòu)。 |
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云輝一鳴
木蟲 (正式寫手)
- 翻譯EPI: 1
- 應(yīng)助: 79 (初中生)
- 金幣: 1811.7
- 散金: 27
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- 在線: 304.7小時
- 蟲號: 2353824
- 注冊: 2013-03-17
- 性別: GG
- 專業(yè): 發(fā)展社會學
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
phu_grassman: 金幣-10, 多次機器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
phu_grassman: 金幣-10, 多次機器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
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全文的翻譯的大概是這樣的 Fiber raw material is the most abundant on earth now, cheap the important renewable resources, and the application of it has always been the focus of the scientific community and hotspot of research. Cellulase is a highly active biocatalyst. With the development of modern microbiological techniques, cellulase in the feed, alcohol, textile and food etc showed the huge market potential. Laboratory research on double function of cellulose enzyme CbCel9A1CBM each module function study found its truncated proteins between the progressive and collaborative. Concrete from the relationship of structure - function for further understanding the truncated protein, we want to be able to CbCel 9 and L - a - L CBM3C module proteins in crystal structure analysis, and to fundamentally understand its action mechanism. First of all, we have to target proteins in Ni - column affinity purification, and found in a molecular sieve chromatography purification purification effect is very good. Based on sds-page gel electrophoresis after after a textual research on the purity of target protein with coomassie brilliant blue method of target protein concentrations were determined, and at the same time, concentrated protein concentration. After diffusion method using sessile drop type steam after a large number of experiments, the optimal target protein crystallization conditions were determined and the cultivation of single crystal. Finally concentrated to Shanghai light source of Shanghai institute of applied physics, Chinese academy of science for X-ray synchrotron radiation diffraction resolution crystal structure. |
2樓2013-05-31 13:36:49
fjtony163
版主 (文壇精英)
米米
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- 管轄: 論文翻譯
3樓2013-05-31 15:48:08
wbcui
至尊木蟲 (知名作家)
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- 專業(yè): 金屬功能材料
【答案】應(yīng)助回帖
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我來翻一下第一段吧，不是這個專業(yè)的，第二段很多專業(yè)名詞都不明白 raw fiber materials are the most enriched and important resource upt to now, which is also considered to be recyclable and low-cost. Many emphasis and attractions were paid to their applications in this field. fiber enzyme is a kind of highly activated biocaltalyst. with the developement of modern microbiotechnology, giant maketing potentials of fiber catalysts have appeared in the fields of fodders, alcohol, textile and food. 很多專業(yè)名詞不是很懂，拋磚引玉吧 |
4樓2013-05-31 23:00:19
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