 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 4 | 1/1 | 返回列表 |
| 查看: 888 | 回復(fù): 3 | |||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | |||
jiangbo9090木蟲(chóng) (小有名氣)
|
[求助]
摘要翻譯
|
||
|
纖維原料是地球上現(xiàn)今最豐富、可再生的廉價(jià)的重要資源，對(duì)它的應(yīng)用的研究一直是科學(xué)界的重點(diǎn)和熱點(diǎn)。纖維素酶是一種高活性生物催化劑。隨著現(xiàn)代微生物技術(shù)的發(fā)展，纖維素酶在飼料、酒精、紡織和食品等領(lǐng)域顯現(xiàn)出巨大的市場(chǎng)潛力。 實(shí)驗(yàn)室課題組對(duì)雙功能纖維素酶CbCel9A1CBM各模塊功能研究后發(fā)現(xiàn)其各截短蛋白間存在漸進(jìn)性和協(xié)同性。為進(jìn)一步具體從結(jié)構(gòu)-功能的關(guān)系了解各截短蛋白，我們希望能夠?qū)bCel 9A-L和L-CBM3C模塊蛋白經(jīng)行晶體結(jié)構(gòu)的解析，從而能從根本上了解其作用機(jī)理。首先，我們對(duì)目標(biāo)蛋白經(jīng)行了Ni-柱親和分離純化，并通過(guò)分子篩層析純化發(fā)現(xiàn)純化效果很好。之后根據(jù)SDS-PAGE凝膠電泳對(duì)目標(biāo)蛋白的純度進(jìn)行了考證后用考馬斯亮藍(lán)法對(duì)目標(biāo)蛋白的濃度進(jìn)行了測(cè)定，并同時(shí)濃縮蛋白濃度。之后用懸滴式蒸汽擴(kuò)散法進(jìn)行大量實(shí)驗(yàn)后，確定了目標(biāo)蛋白的最優(yōu)結(jié)晶條件并進(jìn)行了單晶培養(yǎng)。最后集中送往上海光源中國(guó)科學(xué)院上海應(yīng)用物理研究所進(jìn)行X-光同步輻射衍射解析晶體結(jié)構(gòu)。 |
回復(fù)此樓» 猜你喜歡
一志愿哈爾濱工業(yè)大學(xué)材料與化工方向336分
已經(jīng)有5人回復(fù)
-
0856材料化工調(diào)劑 總分330
已經(jīng)有9人回復(fù)
-
085602 化工專(zhuān)碩 338分 求調(diào)劑
已經(jīng)有9人回復(fù)
-
352分 化工與材料
已經(jīng)有4人回復(fù)
-
求調(diào)劑
已經(jīng)有8人回復(fù)
-
材料求調(diào)劑 一志愿哈工大總分298分，前三科223分
已經(jīng)有3人回復(fù)
-
材料求調(diào)劑一志愿哈工大324
已經(jīng)有4人回復(fù)
-
085600 286分 材料求調(diào)劑
已經(jīng)有4人回復(fù)
-
求調(diào)劑
已經(jīng)有7人回復(fù)
-
275求調(diào)劑
已經(jīng)有10人回復(fù)
云輝一鳴
木蟲(chóng) (正式寫(xiě)手)
- 翻譯EPI: 1
- 應(yīng)助: 79 (初中生)
- 金幣: 1811.7
- 散金: 27
- 紅花: 5
- 帖子: 465
- 在線(xiàn): 304.7小時(shí)
- 蟲(chóng)號(hào): 2353824
- 注冊(cè): 2013-03-17
- 性別: GG
- 專(zhuān)業(yè): 發(fā)展社會(huì)學(xué)
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
phu_grassman: 金幣-10, 多次機(jī)器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
phu_grassman: 金幣-10, 多次機(jī)器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
|
全文的翻譯的大概是這樣的 Fiber raw material is the most abundant on earth now, cheap the important renewable resources, and the application of it has always been the focus of the scientific community and hotspot of research. Cellulase is a highly active biocatalyst. With the development of modern microbiological techniques, cellulase in the feed, alcohol, textile and food etc showed the huge market potential. Laboratory research on double function of cellulose enzyme CbCel9A1CBM each module function study found its truncated proteins between the progressive and collaborative. Concrete from the relationship of structure - function for further understanding the truncated protein, we want to be able to CbCel 9 and L - a - L CBM3C module proteins in crystal structure analysis, and to fundamentally understand its action mechanism. First of all, we have to target proteins in Ni - column affinity purification, and found in a molecular sieve chromatography purification purification effect is very good. Based on sds-page gel electrophoresis after after a textual research on the purity of target protein with coomassie brilliant blue method of target protein concentrations were determined, and at the same time, concentrated protein concentration. After diffusion method using sessile drop type steam after a large number of experiments, the optimal target protein crystallization conditions were determined and the cultivation of single crystal. Finally concentrated to Shanghai light source of Shanghai institute of applied physics, Chinese academy of science for X-ray synchrotron radiation diffraction resolution crystal structure. |
2樓2013-05-31 13:36:49
fjtony163
版主 (文壇精英)
米米
- 翻譯EPI: 386
- 應(yīng)助: 361 (碩士)
- 貴賓: 6.876
- 金幣: 70610.6
- 散金: 36050
- 紅花: 222
- 沙發(fā): 616
- 帖子: 21912
- 在線(xiàn): 3793.7小時(shí)
- 蟲(chóng)號(hào): 1397876
- 注冊(cè): 2011-09-11
- 專(zhuān)業(yè): 遺傳學(xué)研究新技術(shù)與方法
- 管轄: 論文翻譯
3樓2013-05-31 15:48:08
wbcui
至尊木蟲(chóng) (知名作家)
- 翻譯EPI: 29
- 應(yīng)助: 221 (大學(xué)生)
- 貴賓: 0.349
- 金幣: 16296.6
- 散金: 359
- 紅花: 73
- 帖子: 8321
- 在線(xiàn): 919小時(shí)
- 蟲(chóng)號(hào): 543804
- 注冊(cè): 2008-04-11
- 專(zhuān)業(yè): 金屬功能材料
【答案】應(yīng)助回帖
|
我來(lái)翻一下第一段吧，不是這個(gè)專(zhuān)業(yè)的，第二段很多專(zhuān)業(yè)名詞都不明白 raw fiber materials are the most enriched and important resource upt to now, which is also considered to be recyclable and low-cost. Many emphasis and attractions were paid to their applications in this field. fiber enzyme is a kind of highly activated biocaltalyst. with the developement of modern microbiotechnology, giant maketing potentials of fiber catalysts have appeared in the fields of fodders, alcohol, textile and food. 很多專(zhuān)業(yè)名詞不是很懂，拋磚引玉吧 |
4樓2013-05-31 23:00:19
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 jiangbo9090 的主題更新
| 4 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 320分，材料與化工專(zhuān)業(yè)，求調(diào)劑 +5 | 一定上岸aaa 2026-03-27 | 7/350 |
|
|---|---|---|---|---|
|
[考研] 295材料工程專(zhuān)碩求調(diào)劑 +5 | 1428151015 2026-03-27 | 5/250 |
|
|
|
[考研] 化學(xué)調(diào)劑 +4 | 愛(ài)吃番茄的旭 2026-03-24 | 5/250 |
|
|
|
[考研] 考研調(diào)劑 +4 | Sanmu-124 2026-03-26 | 4/200 |
|
|
|
[考研] 272求調(diào)劑 +7 | 腳滑的守法公民 2026-03-27 | 7/350 |
|
|
|
[考研] 0703化學(xué) +6 | 妮妮ninicgb 2026-03-27 | 6/300 |
|
|
|
[考研] 308求調(diào)劑 +6 | 墨墨漠 2026-03-27 | 6/300 |
|
|
|
[考研] 298調(diào)劑 +3 | jiyingjie123 2026-03-27 | 3/150 |
|
|
|
[考研] 調(diào)劑推薦 +5 | 清酒714 2026-03-26 | 6/300 |
|
|
|
[考研] 材料學(xué)碩333求調(diào)劑 +8 | 北道巷 2026-03-24 | 8/400 |
|
|
|
[考研] 304求調(diào)劑 +3 | 曼殊2266 2026-03-27 | 3/150 |
|
|
|
[考研] 351求調(diào)劑 +4 | 麥克阿磊 2026-03-24 | 4/200 |
|
|
|
[考研] 336材料求調(diào)劑 +7 | 陳瀅瑩 2026-03-26 | 9/450 |
|
|
|
[考研] 一志愿天津大學(xué)339材料與化工求調(diào)劑 +3 | 江往賣(mài)魚(yú) 2026-03-26 | 3/150 |
|
|
|
[考研] 一志愿上海交大生物與醫(yī)藥專(zhuān)碩324分，求調(diào)劑 +6 | jiajunX 2026-03-22 | 6/300 |
|
|
|
[考研] 【2026考研調(diào)劑】制藥工程 284分 求相關(guān)專(zhuān)業(yè)調(diào)劑名額 +4 | 袁奐奐 2026-03-25 | 8/400 |
|
|
|
[考研] 347求調(diào)劑 +4 | L when 2026-03-25 | 4/200 |
|
|
|
[考研] 306求0703調(diào)劑一志愿華中師范 +10 | 紙魚(yú)ly 2026-03-21 | 11/550 |
|
|
|
[考研] 080500求調(diào)劑 +3 | zzzzfan 2026-03-24 | 3/150 |
|
|
|
[考研] 求老師收我 +3 | zzh16938784 2026-03-23 | 3/150 |
|