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| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | |||
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jiangbo9090木蟲 (小有名氣)
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[求助]
摘要翻譯
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纖維原料是地球上現(xiàn)今最豐富、可再生的廉價(jià)的重要資源，對(duì)它的應(yīng)用的研究一直是科學(xué)界的重點(diǎn)和熱點(diǎn)。纖維素酶是一種高活性生物催化劑。隨著現(xiàn)代微生物技術(shù)的發(fā)展，纖維素酶在飼料、酒精、紡織和食品等領(lǐng)域顯現(xiàn)出巨大的市場(chǎng)潛力。 實(shí)驗(yàn)室課題組對(duì)雙功能纖維素酶CbCel9A1CBM各模塊功能研究后發(fā)現(xiàn)其各截短蛋白間存在漸進(jìn)性和協(xié)同性。為進(jìn)一步具體從結(jié)構(gòu)-功能的關(guān)系了解各截短蛋白，我們希望能夠?qū)bCel 9A-L和L-CBM3C模塊蛋白經(jīng)行晶體結(jié)構(gòu)的解析，從而能從根本上了解其作用機(jī)理。首先，我們對(duì)目標(biāo)蛋白經(jīng)行了Ni-柱親和分離純化，并通過(guò)分子篩層析純化發(fā)現(xiàn)純化效果很好。之后根據(jù)SDS-PAGE凝膠電泳對(duì)目標(biāo)蛋白的純度進(jìn)行了考證后用考馬斯亮藍(lán)法對(duì)目標(biāo)蛋白的濃度進(jìn)行了測(cè)定，并同時(shí)濃縮蛋白濃度。之后用懸滴式蒸汽擴(kuò)散法進(jìn)行大量實(shí)驗(yàn)后，確定了目標(biāo)蛋白的最優(yōu)結(jié)晶條件并進(jìn)行了單晶培養(yǎng)。最后集中送往上海光源中國(guó)科學(xué)院上海應(yīng)用物理研究所進(jìn)行X-光同步輻射衍射解析晶體結(jié)構(gòu)。 |
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wbcui
至尊木蟲 (知名作家)
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【答案】應(yīng)助回帖
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我來(lái)翻一下第一段吧，不是這個(gè)專業(yè)的，第二段很多專業(yè)名詞都不明白 raw fiber materials are the most enriched and important resource upt to now, which is also considered to be recyclable and low-cost. Many emphasis and attractions were paid to their applications in this field. fiber enzyme is a kind of highly activated biocaltalyst. with the developement of modern microbiotechnology, giant maketing potentials of fiber catalysts have appeared in the fields of fodders, alcohol, textile and food. 很多專業(yè)名詞不是很懂，拋磚引玉吧 |
4樓2013-05-31 23:00:19
云輝一鳴
木蟲 (正式寫手)
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
phu_grassman: 金幣-10, 多次機(jī)器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
phu_grassman: 金幣-10, 多次機(jī)器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
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全文的翻譯的大概是這樣的 Fiber raw material is the most abundant on earth now, cheap the important renewable resources, and the application of it has always been the focus of the scientific community and hotspot of research. Cellulase is a highly active biocatalyst. With the development of modern microbiological techniques, cellulase in the feed, alcohol, textile and food etc showed the huge market potential. Laboratory research on double function of cellulose enzyme CbCel9A1CBM each module function study found its truncated proteins between the progressive and collaborative. Concrete from the relationship of structure - function for further understanding the truncated protein, we want to be able to CbCel 9 and L - a - L CBM3C module proteins in crystal structure analysis, and to fundamentally understand its action mechanism. First of all, we have to target proteins in Ni - column affinity purification, and found in a molecular sieve chromatography purification purification effect is very good. Based on sds-page gel electrophoresis after after a textual research on the purity of target protein with coomassie brilliant blue method of target protein concentrations were determined, and at the same time, concentrated protein concentration. After diffusion method using sessile drop type steam after a large number of experiments, the optimal target protein crystallization conditions were determined and the cultivation of single crystal. Finally concentrated to Shanghai light source of Shanghai institute of applied physics, Chinese academy of science for X-ray synchrotron radiation diffraction resolution crystal structure. |
2樓2013-05-31 13:36:49
fjtony163
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米米
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