 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 891 | 回復(fù): 3 | ||
| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | ||
| 當(dāng)前只顯示滿足指定條件的回帖，點擊這里查看本話題的所有回帖 | ||
jiangbo9090木蟲 (小有名氣)
|
[求助]
摘要翻譯
|
|
|
纖維原料是地球上現(xiàn)今最豐富、可再生的廉價的重要資源，對它的應(yīng)用的研究一直是科學(xué)界的重點和熱點。纖維素酶是一種高活性生物催化劑。隨著現(xiàn)代微生物技術(shù)的發(fā)展，纖維素酶在飼料、酒精、紡織和食品等領(lǐng)域顯現(xiàn)出巨大的市場潛力。 實驗室課題組對雙功能纖維素酶CbCel9A1CBM各模塊功能研究后發(fā)現(xiàn)其各截短蛋白間存在漸進性和協(xié)同性。為進一步具體從結(jié)構(gòu)-功能的關(guān)系了解各截短蛋白，我們希望能夠?qū)bCel 9A-L和L-CBM3C模塊蛋白經(jīng)行晶體結(jié)構(gòu)的解析，從而能從根本上了解其作用機理。首先，我們對目標(biāo)蛋白經(jīng)行了Ni-柱親和分離純化，并通過分子篩層析純化發(fā)現(xiàn)純化效果很好。之后根據(jù)SDS-PAGE凝膠電泳對目標(biāo)蛋白的純度進行了考證后用考馬斯亮藍法對目標(biāo)蛋白的濃度進行了測定，并同時濃縮蛋白濃度。之后用懸滴式蒸汽擴散法進行大量實驗后，確定了目標(biāo)蛋白的最優(yōu)結(jié)晶條件并進行了單晶培養(yǎng)。最后集中送往上海光源中國科學(xué)院上海應(yīng)用物理研究所進行X-光同步輻射衍射解析晶體結(jié)構(gòu)。 |
回復(fù)此樓» 猜你喜歡
299求調(diào)劑
已經(jīng)有8人回復(fù)
-
一志愿北京理工大學(xué)本科211材料工程294求調(diào)劑
已經(jīng)有6人回復(fù)
-
300求調(diào)劑，材料科學(xué)英一數(shù)二
已經(jīng)有8人回復(fù)
-
招收生物學(xué)/細胞生物學(xué)調(diào)劑
已經(jīng)有5人回復(fù)
-
070305高分子化學(xué)與物理 304分求調(diào)劑
已經(jīng)有7人回復(fù)
-
289求調(diào)劑
已經(jīng)有13人回復(fù)
-
一志愿哈爾濱工業(yè)大學(xué)材料與化工方向336分
已經(jīng)有9人回復(fù)
-
081200-11408-276學(xué)碩求調(diào)劑
已經(jīng)有6人回復(fù)
-
調(diào)劑求院校招收
已經(jīng)有5人回復(fù)
-
調(diào)劑310
已經(jīng)有8人回復(fù)
fjtony163
版主 (文壇精英)
米米
- 翻譯EPI: 386
- 應(yīng)助: 361 (碩士)
- 貴賓: 6.876
- 金幣: 70610.6
- 散金: 36050
- 紅花: 222
- 沙發(fā): 616
- 帖子: 21912
- 在線: 3793.7小時
- 蟲號: 1397876
- 注冊: 2011-09-11
- 專業(yè): 遺傳學(xué)研究新技術(shù)與方法
- 管轄: 論文翻譯
3樓2013-05-31 15:48:08
云輝一鳴
木蟲 (正式寫手)
- 翻譯EPI: 1
- 應(yīng)助: 79 (初中生)
- 金幣: 1811.7
- 散金: 27
- 紅花: 5
- 帖子: 465
- 在線: 304.7小時
- 蟲號: 2353824
- 注冊: 2013-03-17
- 性別: GG
- 專業(yè): 發(fā)展社會學(xué)
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
phu_grassman: 金幣-10, 多次機器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
phu_grassman: 金幣-10, 多次機器翻譯。 2013-05-31 16:43:13
jiangbo9090: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 2013-06-01 07:56:11
|
全文的翻譯的大概是這樣的 Fiber raw material is the most abundant on earth now, cheap the important renewable resources, and the application of it has always been the focus of the scientific community and hotspot of research. Cellulase is a highly active biocatalyst. With the development of modern microbiological techniques, cellulase in the feed, alcohol, textile and food etc showed the huge market potential. Laboratory research on double function of cellulose enzyme CbCel9A1CBM each module function study found its truncated proteins between the progressive and collaborative. Concrete from the relationship of structure - function for further understanding the truncated protein, we want to be able to CbCel 9 and L - a - L CBM3C module proteins in crystal structure analysis, and to fundamentally understand its action mechanism. First of all, we have to target proteins in Ni - column affinity purification, and found in a molecular sieve chromatography purification purification effect is very good. Based on sds-page gel electrophoresis after after a textual research on the purity of target protein with coomassie brilliant blue method of target protein concentrations were determined, and at the same time, concentrated protein concentration. After diffusion method using sessile drop type steam after a large number of experiments, the optimal target protein crystallization conditions were determined and the cultivation of single crystal. Finally concentrated to Shanghai light source of Shanghai institute of applied physics, Chinese academy of science for X-ray synchrotron radiation diffraction resolution crystal structure. |
2樓2013-05-31 13:36:49
wbcui
至尊木蟲 (知名作家)
- 翻譯EPI: 29
- 應(yīng)助: 221 (大學(xué)生)
- 貴賓: 0.349
- 金幣: 16296.6
- 散金: 359
- 紅花: 73
- 帖子: 8321
- 在線: 919小時
- 蟲號: 543804
- 注冊: 2008-04-11
- 專業(yè): 金屬功能材料
【答案】應(yīng)助回帖
|
我來翻一下第一段吧，不是這個專業(yè)的，第二段很多專業(yè)名詞都不明白 raw fiber materials are the most enriched and important resource upt to now, which is also considered to be recyclable and low-cost. Many emphasis and attractions were paid to their applications in this field. fiber enzyme is a kind of highly activated biocaltalyst. with the developement of modern microbiotechnology, giant maketing potentials of fiber catalysts have appeared in the fields of fodders, alcohol, textile and food. 很多專業(yè)名詞不是很懂，拋磚引玉吧 |
4樓2013-05-31 23:00:19
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 求調(diào)劑 +6 | 蘆lty 2026-03-25 | 7/350 |
|
|---|---|---|---|---|
|
|
[考研] 張芳銘-中國農(nóng)業(yè)大學(xué)-環(huán)境工程專碩-298 +4 | 手機用戶 2026-03-26 | 4/200 |
|
|
[考研] 085600，材料與化工321分調(diào)劑 +4 | 大饞小子 2026-03-27 | 6/300 |
|
|
|
[考研] 化學(xué)308分求調(diào)劑 +8 | 你好明天你好 2026-03-23 | 9/450 |
|
|
|
[考研] 求調(diào)劑 +3 | 劉柯@ 2026-03-24 | 4/200 |
|
|
|
[論文投稿] Journal of Mechanical Science and Technology +3 | Russ_ss 2026-03-25 | 5/250 |
|
|
|
[考研] 一志愿陜師大生物學(xué)071000，298分，求調(diào)劑 +5 | SYA！ 2026-03-23 | 5/250 |
|
|
|
[考研] 324求調(diào)劑 +8 | hanamiko 2026-03-26 | 10/500 |
|
|
|
[考研]
 304材料求調(diào)劑
+4
|
鐘llll 2026-03-26 | 4/200 |
|
|
|
[考研] 調(diào)劑求收留 +7 | 果然有我 2026-03-26 | 7/350 |
|
|
|
[考研] 294分080500材料科學(xué)與工程求調(diào)劑 +4 | 柳溪邊 2026-03-26 | 4/200 |
|
|
|
[考研] 327求調(diào)劑 +7 | prayer13 2026-03-23 | 7/350 |
|
|
|
[考研]
材料調(diào)劑
 5+4
|
想要一壺桃花水 2026-03-25 | 10/500 |
|
|
|
[考研] 081700 調(diào)劑 267分 +11 | 迷人的哈哈 2026-03-23 | 11/550 |
|
|
|
[考研] 環(huán)境專碩324分求調(diào)劑推薦 +5 | 軒小寧—— 2026-03-26 | 5/250 |
|
|
|
[考研] 334分 一志愿武理-080500 材料求調(diào)劑 +4 | 李李不服輸 2026-03-25 | 4/200 |
|
|
|
[考研] 0854電子信息求調(diào)劑 +7 | α____ 2026-03-22 | 9/450 |
|
|
|
[考研] 347求調(diào)劑 +4 | L when 2026-03-25 | 4/200 |
|
|
|
[考研] 求調(diào)劑 +6 | 研研，接電話 2026-03-24 | 7/350 |
|
|
|
[考研] 277分求調(diào)劑，跨調(diào)材料 +3 | 考研調(diào)劑lxh 2026-03-24 | 3/150 |
|