 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 2 | 1/1 | 返回列表 |
| 查看: 855 | 回復(fù): 1 | |||
zhongjin1988新蟲 (初入文壇)
|
[求助]
β半乳糖苷酶的酶活定量實(shí)驗(yàn)步驟求助
|
|
請(qǐng)問各位大俠： 有沒有誰做過β半乳糖苷酶的酶活定量實(shí)驗(yàn)��？我想做一下這個(gè)酶的酶活定量十余年，可是不知道怎么做啊，要用什么藥劑，要具體步驟，急，求助！~~~ 謝謝啦 |
回復(fù)此樓» 收錄本帖的淘帖專輯推薦
 分子生化實(shí)驗(yàn)經(jīng)驗(yàn)積累 |
» 猜你喜歡
不合理蛙科研實(shí)驗(yàn)中的趣事：實(shí)驗(yàn)器材的 “烏龍”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)中的趣事：和實(shí)驗(yàn)材料的 “斗智斗勇”
已經(jīng)有0人回復(fù)
-
化學(xué)工程及工業(yè)化學(xué)論文潤(rùn)色/翻譯怎么收費(fèi)?
已經(jīng)有75人回復(fù)
-
蛋白質(zhì)檢測(cè)：精準(zhǔn)分析，解鎖生物分子的密碼
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之小鼠實(shí)驗(yàn)：嚴(yán)謹(jǐn)設(shè)計(jì)，解析生命機(jī)制的重要載體
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之重金屬檢測(cè)：精準(zhǔn)篩查，守護(hù)健康與環(huán)境的防線
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之“蛙測(cè)重金屬我背鍋三千”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實(shí)驗(yàn)之“鼠逃三次我發(fā)三篇SCI”
已經(jīng)有0人回復(fù)
-
納米粒度分析
已經(jīng)有3人回復(fù)
-
林科院林化所招收材料與化工專業(yè)碩士，坐標(biāo)南京
已經(jīng)有0人回復(fù)
» 本主題相關(guān)價(jià)值貼推薦，對(duì)您同樣有幫助:
- 酶標(biāo)儀測(cè)定酶活
已經(jīng)有10人回復(fù)
- 酶活 比酶活
已經(jīng)有8人回復(fù)
- 求助細(xì)胞內(nèi)活性氧定量檢測(cè)實(shí)驗(yàn)方法！
已經(jīng)有18人回復(fù)
- 測(cè)定β-半乳糖苷酶酶活為什么重復(fù)性差？？求高人指點(diǎn)
已經(jīng)有17人回復(fù)
- β-糖苷酶表達(dá)可溶性蛋白很多，怎么就是沒有活性
已經(jīng)有24人回復(fù)
- 殼聚糖固定化酶，沒有活力。求原因
已經(jīng)有8人回復(fù)
- 果膠酶活力測(cè)定
已經(jīng)有10人回復(fù)
- 測(cè)定β-半乳糖苷酶活性用的質(zhì)粒
已經(jīng)有4人回復(fù)
- 【求助/交流】纖維素酶問題
已經(jīng)有16人回復(fù)
- 【求助/交流】基因工程菌誘導(dǎo)蛋白表達(dá)的問題
已經(jīng)有14人回復(fù)
- 【求助/交流】微生物纖維素酶活測(cè)定
已經(jīng)有11人回復(fù)
- 纖維素酶濾紙酶活為什么比內(nèi)切葡聚糖酶��？
已經(jīng)有6人回復(fù)
xyw551268
新蟲 (初入文壇)
- 應(yīng)助: 1 (幼兒園)
- 金幣: 4.8
- 帖子: 2
- 在線: 5.3小時(shí)
- 蟲號(hào): 133096
- 注冊(cè): 2005-12-13
- 性別: GG
- 專業(yè): 系統(tǒng)生理學(xué)
【答案】應(yīng)助回帖
★ ★
感謝參與，應(yīng)助指數(shù) +1
zhongjin1988(gyesang代發(fā)): 金幣+2, 鼓勵(lì)回帖應(yīng)助！ 2013-06-03 12:43:25
感謝參與，應(yīng)助指數(shù) +1
zhongjin1988(gyesang代發(fā)): 金幣+2, 鼓勵(lì)回帖應(yīng)助！ 2013-06-03 12:43:25
|
Cell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity Posted on Monday, October 27, 2003 Description Cell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity Procedure 1. Carefully remove the growth media from the cell culture. 2. Add an equal volume of PBS and gently mix the PBS solution around to wash out the cell culture media. 3. Repeat the PBS wash of the cells two more times. 4. Discard the PBS wash solution. 5. Add Fixative Solution to the cells and incubate at room temperature for 3 to 5 min (see Hint #2). 6. Wash cells as before in PBS two times. 7. Add between 1 to 2 ml of Staining Solution per 35 mm culture dish or cover slide. 8. Incubate for at least 2 hr at 37°C (do not incubate in a CO2 incubator; see Hint #3). Recipes Staining Solution 1 ml of 100 mM K3Fe(CN)6 (final concentration 5 mM) 40 ìl of 1 M MgCl2 (final concentration 2 mM) *Add just before use 600 ìl of 5 M NaCl (final concentration 150 mM) Prepare Staining Solution the day of experiment 4 ml of Citric Acid/Phosphate Buffer (final concentration 30 mM) 1 ml of 100 mM K4Fe(CN)6 (final concentration 5 mM) 1 ml of X-Gal Solution* (final concentration 1 mg/ml) Cell Fixative in PBS 2% (v/v) Formaldehyde 0.2% (w/v) Glutaraldehyde 1 M MgCl2 5 M NaCl X-Gal Solution in DMF (CAUTION! see Hint #1) 20 mg/ml X-Gal (5-bromo-4-chloro-3-indoly Â-D-galactopyranoside) Store -20°C X-Gal is not stable in water and should be added to the Staining Solution just before use 100 mM Potassium Ferricyanide 100 mM Potassium Ferricyanide (K3Fe(CN)6) CAUTION see Hint #1 100 mM Potassium Ferrocyanide 100 mM Potassium Ferrocyanide (K4Fe(CN)6) CAUTION see Hint #1 Citric Acid/Phosphate Buffer 0.1 M Citric Acid 0.2 M Sodium Phosphate Dibasic (Na2HPO4) Proper pH is VERY important pH 6.0 PBS pH 7.2 2.7 mM KCl 4.3 mM Sodium Phosphate Dibasic (Na2HPO4) 1.8 mM Potassium Phosphate Monobasic (KH2PO4) 137 mM NaCl Supplies Tips 1. CAUTION! This substance is a biohazard. Consult this agent's MSDS for proper handling instructions. 2. Cells can also be fixed in 3% (v/v) Formaldehyde in PBS; however the Fixative Solution preserves morphology better. 3. A blue color is visible within 2 hr; however, staining is maximum at between 12 to 16 hr. Blue stain is indicative of senescence-associated â-galactosidase activity. |
2樓2013-06-03 04:39:47
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 zhongjin1988 的主題更新
| 2 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研]
 085602 289分求調(diào)劑
+3
|
WWW西西弗斯 2026-03-24 | 3/150 |
|
|---|---|---|---|---|
|
|
[考研] 材料調(diào)劑 +5 | 匹克i 2026-03-23 | 5/250 |
|
|
[考研] 一志愿南航材料專317分求調(diào)劑 +4 | 炸呀炸呀炸薯?xiàng)l 2026-03-23 | 4/200 |
|
|
|
[考研] 07化學(xué)280分求調(diào)劑 +4 | 722865 2026-03-23 | 4/200 |
|
|
|
[考研] 材料專碩找調(diào)劑 +3 | 哈哈哈吼吼吼哈 2026-03-23 | 3/150 |
|
|
|
[考研] 一志愿國(guó)科過程所081700，274求調(diào)劑 +3 | 三水研0水立方 2026-03-23 | 3/150 |
|
|
|
[考研] 一志愿中南大學(xué)化學(xué)學(xué)碩0703總分337求調(diào)劑 +5 | niko- 2026-03-22 | 5/250 |
|
|
|
[考研] 0703化學(xué)求調(diào)劑 +4 | 奶油草莓. 2026-03-22 | 5/250 |
|
|
|
[考研] 考研化學(xué)308分求調(diào)劑 +7 | 你好明天你好 2026-03-23 | 8/400 |
|
|
|
[考研] 生物學(xué)一志愿985，分?jǐn)?shù)349求調(diào)劑 +6 | zxts12 2026-03-21 | 9/450 |
|
|
|
[考研] 一志愿南京理工大學(xué)085701資源與環(huán)境302分求調(diào)劑 +5 | 葵梓衛(wèi)隊(duì) 2026-03-18 | 7/350 |
|
|
|
[考研] 352求調(diào)劑 +3 | 大米飯！ 2026-03-22 | 3/150 |
|
|
|
[考研] 324求調(diào)劑 +6 | lucky呀呀呀鴨 2026-03-20 | 6/300 |
|
|
|
[考研] 313求調(diào)劑 +4 | 肆叁貳壹22 2026-03-19 | 4/200 |
|
|
|
[考研] 材料 271求調(diào)劑 +5 | 展信悅_ 2026-03-21 | 5/250 |
|
|
|
[考研] 一志愿西南交大，求調(diào)劑 +5 | 材化逐夢(mèng)人 2026-03-18 | 5/250 |
|
|
|
[考研] 296求調(diào)劑 +6 | www_q 2026-03-18 | 10/500 |
|
|
|
[考研] 086500 325 求調(diào)劑 +3 | 領(lǐng)帶小熊 2026-03-19 | 3/150 |
|
|
|
[考研] 招收調(diào)劑碩士 +4 | lidianxing 2026-03-19 | 12/600 |
|
|
|
[考研] 材料考研調(diào)劑 +3 | xwt。 2026-03-19 | 3/150 |
|