| 5 | 1/1 | 返回列表 |
| 查看: 1940 | 回復(fù): 4 | |||
| 當(dāng)前只顯示滿足指定條件的回帖,點擊這里查看本話題的所有回帖 | |||
yuehedou木蟲 (小有名氣)
|
[求助]
miRNA高通量測序分析時拷貝數(shù)的選取問題
|
||
|
分析高通量測序的miRNA數(shù)據(jù)時,通過質(zhì)控的序列在進入后續(xù)分析(conserved,novel等的分析預(yù)測)時,對拷貝數(shù)(count數(shù))有沒有要求? 我在文獻中看到有人全部拿去分析,有人去除了單拷貝,還有人甚至只用10拷貝以上的序列。 歡迎指教、討論! |
miRNA測序數(shù)據(jù)分析 |

|
miRBase有這樣的建議: (1) Multiple reads (10–20 are commonly used cutoffs) support the presence of the mature microRNA (preferably from multiple independent experiments); (2) The reads map to an extended sequence region (e.g. an assembled contig), and the sequence flanking the putative mature microRNA folds to form a microRNA precursor-like hairpin with strong pairing between the mature microRNA and the opposite arm. Reads that map very many times to a genome sequence should be discarded; (3) Mapped reads do not overlap other annotated transcripts (i.e. there is no evidence that the short readsmay represent fragments of mRNAs or other known RNA types); (4) Reads mapping to a locus support consistent processing of the 5'-end of the mature sequence (for example, the majority of reads overlapping a given mature microRNA annotation should have the same 5'-end; the 3'-end may be significantly more variable); and (5) Ideally, reads will support the presence of mature sequences from both arms of the predicted hairpin (so-called miR and miR* sequences), and the putative mature sequences should base-pair with the correct 3'-overhang. miRBase: integrating microRNA annotation and deep-sequencing data |
金蟲 (小有名氣)
木蟲 (小有名氣)

木蟲 (小有名氣)

| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 調(diào)劑求院校招收 +7 | 鶴鯨鴿 2026-03-28 | 7/350 |
|
|---|---|---|---|---|
|
[考研] 085404 22408 315分 +4 | zhuangyan123 2026-03-31 | 4/200 |
|
|
[考研] 323分 食品與營養(yǎng)調(diào)劑 +3 | 嘿ooo 2026-03-31 | 3/150 |
|
|
[考研] 一志愿廈門大學(xué)材料工程專碩354找調(diào)劑。! +5 | 貝唄鋇鋇 2026-03-30 | 5/250 |
|
|
[考研] 303求調(diào)劑 +7 | DLkz1314. 2026-03-30 | 7/350 |
|
|
[考研] 318求調(diào)劑 +7 | 陳晨79 2026-03-30 | 7/350 |
|
|
[考研] 求調(diào)劑,一志愿 南京航空航天大學(xué)大學(xué) ,080500材料科學(xué)與工程學(xué)碩 +6 | @taotao 2026-03-26 | 7/350 |
|
|
[考研] 0856求調(diào)劑 +8 | 楒桉 2026-03-28 | 8/400 |
|
|
[考研] 一志愿211,335分,0856,求調(diào)劑院校和導(dǎo)師 +7 | 傾____蕭 2026-03-27 | 8/400 |
|
|
[考研] 318一志愿吉林大學(xué)生物與醫(yī)藥 求調(diào)劑 +5 | 篤行致遠. 2026-03-28 | 5/250 |
|
|
[考研] 290求調(diào)劑 +3 | dfffsar 2026-03-29 | 3/150 |
|
|
[考研] 305求調(diào)劑 +8 | RuiFairyrui 2026-03-28 | 8/400 |
|
|
[考研] 330分求調(diào)劑 +5 | qzenlc 2026-03-29 | 5/250 |
|
|
[考研] 071000生物學(xué)求調(diào)劑,初試成績343 +7 | 小小甜面團 2026-03-25 | 7/350 |
|
|
[考研] 壓國家一區(qū)線,求導(dǎo)師收留,有恩必謝! +7 | 迷人的哈哈 2026-03-28 | 7/350 |
|
|
[考研] 085405 考的11408求各位老師帶走 +3 | Qiu學(xué)ing 2026-03-28 | 3/150 |
|
|
[考研] 330一志愿中國海洋大學(xué) 化學(xué)工程 085602 有讀博意愿 求調(diào)劑 +3 | wywy.. 2026-03-27 | 4/200 |
|
|
[考研] 341求調(diào)劑 +7 | 青檸檬1 2026-03-26 | 7/350 |
|
|
[考研] 081200-11408-276學(xué)碩求調(diào)劑 +3 | 崔wj 2026-03-26 | 3/150 |
|
|
[考研] 【2026考研調(diào)劑】制藥工程 284分 求相關(guān)專業(yè)調(diào)劑名額 +4 | 袁奐奐 2026-03-25 | 8/400 |
|