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塵香駐風(fēng)金蟲 (小有名氣)
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[交流]
螢光素酶檢測系統(tǒng) 已有2人參與
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| 有沒有蟲友用過Promega公司的螢光素酶檢測系統(tǒng)試劑盒啊?用的是哪種型號的? |
金蟲 (小有名氣)
金蟲 (著名寫手)
金蟲 (小有名氣)
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請問你用這個檢測的時候是多樣品還是少樣品,我看它說明書上說的是發(fā)光至少持續(xù)一分鐘,那么會不會等加完全部的時候前面加的就開始衰退了呢? |
至尊木蟲 (知名作家)
Translator and Proofreader
送紅花一朵 |
These kind of assays are usually done with a Luminescence Microplate Reader. All you need to do is to prepare your cell lysates and add them to a 96-well plate. Then the machine will read the plate for you. The machine reads well by well in the plate and will inject the Stop & Glo reagents right before reading.... You can do it manually if you can't fine the machine. In this case, you have to add the reagents by yourself and read the samples right away. It's time-consuming though. If you do such experiments as transient transfection and transactivation assays, you need cotransfect a control reporter (Renilla series). That's the reason that you need a dual-luciferase assay system. |
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