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塵香駐風(fēng)金蟲(chóng) (小有名氣)
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[交流]
螢光素酶檢測(cè)系統(tǒng) 已有2人參與
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| 有沒(méi)有蟲(chóng)友用過(guò)Promega公司的螢光素酶檢測(cè)系統(tǒng)試劑盒?用的是哪種型號(hào)的? |
金蟲(chóng) (小有名氣)
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請(qǐng)問(wèn)你用這個(gè)檢測(cè)的時(shí)候是多樣品還是少樣品,我看它說(shuō)明書(shū)上說(shuō)的是發(fā)光至少持續(xù)一分鐘,那么會(huì)不會(huì)等加完全部的時(shí)候前面加的就開(kāi)始衰退了呢? |
金蟲(chóng) (著名寫(xiě)手)
金蟲(chóng) (小有名氣)
至尊木蟲(chóng) (知名作家)
Translator and Proofreader
送紅花一朵 |
These kind of assays are usually done with a Luminescence Microplate Reader. All you need to do is to prepare your cell lysates and add them to a 96-well plate. Then the machine will read the plate for you. The machine reads well by well in the plate and will inject the Stop & Glo reagents right before reading.... You can do it manually if you can't fine the machine. In this case, you have to add the reagents by yourself and read the samples right away. It's time-consuming though. If you do such experiments as transient transfection and transactivation assays, you need cotransfect a control reporter (Renilla series). That's the reason that you need a dual-luciferase assay system. |
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