| 5 | 1/1 | 返回列表 |
| 查看: 667 | 回復: 3 | ||
| 當前只顯示滿足指定條件的回帖,點擊這里查看本話題的所有回帖 | ||
fushxmy金蟲 (正式寫手)
|
[求助]
在已知菌的全基因的情況下,如何利用蛋白一級數(shù)據(jù)直接查蛋白庫(有文獻支持,在附件)
|
|
|
Plamen A. Demirev在AC上的論文Microorganism Identification by Mass Spectrometry and Protein Database Searches,Anal. Chem. 1999, 71, 2732-2738 在搜庫方法里作者用 A search by protein molecular mass (Mr) and based on the set of protein molecular weights in the spectra was carried out in the SwissProt/TrEMBL database (Expasy, Swiss Bioinformatics Institute) using the Sequence Retrieval System (SRSWWW) module at http://expasy.hcuge.ch/srs5/. An interactive window (Alternative Query Form) allows search by a number of classifiers. In this case, we chose averaged protein MW as the primary classifier. We selected a (3 Da MW window, and the only restriction applied in the query was the choice of the “bacteria” protein subset of the database (in an earlier release of SwissPROT the identifier “prokaryota” was also available). Thus, protein identities and organismic sources were tentatively assigned for all peaks from the experimental spectra within the range from 4 to 15 kDa. 他是用maldi tof 做細菌蛋白在4000-10000,不做二級質(zhì)譜,直接搜庫鑒別蛋白,我看了,始終不知道如何搜索 請問大家,他是如何做的 |

金蟲 (正式寫手)

金蟲 (正式寫手)

金蟲 (正式寫手)

| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 南京大學化學調(diào)劑 +4 | 景隨風 2026-03-29 | 7/350 |
|
|---|---|---|---|---|
|
[考研] 一志愿華東師范大學有機化學專業(yè),初試351分,復試被刷求調(diào)劑! +3 | 真名有冰 2026-03-29 | 4/200 |
|
|
[考研] 321求調(diào)劑 +7 | 璞玉~~ 2026-03-25 | 8/400 |
|
|
[考研] 一志愿哈爾濱工業(yè)大學材料與化工方向336分 +9 | 辰沐5211314 2026-03-26 | 9/450 |
|
|
[考研] 數(shù)一英一271專碩(085401)求調(diào)劑,可跨 +7 | 前行必有光 2026-03-28 | 8/400 |
|
|
[考研] 311(085601)求調(diào)劑 +4 | liziyeyeye 2026-03-28 | 4/200 |
|
|
[考研] 壓國家一區(qū)線,求導師收留,有恩必謝! +7 | 迷人的哈哈 2026-03-28 | 7/350 |
|
|
[考研] 化學0703 調(diào)劑 306分 一志愿211 +4 | 26要上岸 2026-03-28 | 4/200 |
|
|
[考研] 347求調(diào)劑 +3 | 山頂見α 2026-03-25 | 3/150 |
|
|
[考研] 311求調(diào)劑 +9 | lin0039 2026-03-26 | 9/450 |
|
|
[考研] 張芳銘-中國農(nóng)業(yè)大學-環(huán)境工程專碩-298 +4 | 手機用戶 2026-03-26 | 4/200 |
|
|
[考研] 272求調(diào)劑 +7 | 腳滑的守法公民 2026-03-27 | 7/350 |
|
|
[考研]
|
18419759900 2026-03-25 | 8/400 |
|
|
[考研] 276求調(diào)劑。有半年電池和半年高分子實習經(jīng)歷 +10 | 材料學257求調(diào)劑 2026-03-23 | 11/550 |
|
|
[考研]
|
鐘llll 2026-03-26 | 4/200 |
|
|
[考研] 321求調(diào)劑 +6 | Ymlll 2026-03-24 | 6/300 |
|
|
[考研] 總分322求生物學/生化與分子/生物信息學相關調(diào)劑 +5 | 星沉uu 2026-03-26 | 6/300 |
|
|
[考研] 284求調(diào)劑 +15 | Zhao anqi 2026-03-22 | 15/750 |
|
|
[考研] 282求調(diào)劑 +3 | wcq131415 2026-03-24 | 3/150 |
|
|
[考研] 求老師收我 +3 | zzh16938784 2026-03-23 | 3/150 |
|