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[交流]
高通量測序中miRNA如何命名?
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我委托一生物公司做甘蔗小RNA高通量測序,生物信息學分析基本過程:過濾后的unique miRNA map到近源物種高粱基因組和甘蔗組裝EST序列。然后與miRBase 數(shù)據(jù)庫中所有植物的miRNA進行比對,給出結果如下;但存在一些疑問:這種以參考miRNA數(shù)據(jù)庫比對得到的植物miRNA進行命名,結果較為復雜。有何種方法命名成像一些文章中所述結果那樣?如按照物種全部統(tǒng)一命名為:ssp164a,ssp164b....,而不是像本結果中分別hit不同物種的164a,最后以不同物種的miRNA進行羅列。 miR_name miR_seq #mir/MIR mirIDs, sbi-miR164a TGGAGAAGCAGGGCACGTGCA 1 sbi-MIR164d, sbi-miR164a TGGAGAAGCAGGGCACGTGCA 1 sbi-MIR164a, sbi-MIR164a-p3 ATGGTTCTTCATGTGCCCATC 1 sbi-MIR164a, zma-miR164a-5p_1ss21AG TGGAGAAGCAGGGCACGTGCG 2 zma-MIR164b,osa-MIR164b, 備注:The miR_name is composed of the 1st known miR name in a cluster, a underscore, and a matching annotation: such as L-n means the miRNA_seq (detected) is n base less than known rep_miRSeq in the left side; R-n means the miRNA_seq (detected) is n base less than known rep_miRSeq in the right side; L+n means the miRNA_seq (detected) is n base more than known rep_miRSeq in the left side; R+n means the miRNA_seq (detected) is n base more than known rep_miRSeq in the right side; 2ss5TC13TA means 2 substitutin (ss), which are T->C at position 5 and T->A at position 13 if there is no matching annotation, the miRNA_seq (detected) is exactly same as known rep_miRSeq. New discovered 5p/3p sequence has been annotated as p3/p5: which is directly differentiate with the reported sequences, |
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