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風(fēng)云uu銅蟲 (小有名氣)
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[求助]
求潤(rùn)色一段話 ,謝謝。。。。。。。。。。
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An ideal cellular probe for practical application should minimally perturb living systems at the concentrations employed. Accordingly, the cytotoxicity of solvent INR1 and INR2 were determined using the MTT assay. In general, at a compound concentration of 5 μM, cellular viability was estimated as greater than 90% after incubation for 2 h (Fig. S3), and higher concentration (10μM) can result in decreased cell survival, 76% for INR1 and 85% for INR2. The results indicate that the probes generally present low toxicity for living cell imaging under the conditions applied. [ Last edited by zhtear99 on 2013-5-13 at 20:08 ] |

鐵桿木蟲 (職業(yè)作家)
院士

金蟲 (正式寫手)
| The ideal cellular probe which is used in practice is suggested to interfere with living systems at a certain concentration. Therefore we measured the quantities of cytotoxicity factor ,namely INR1 and INR2, by using MTT assay. After incubates 2h, the cellular viability is usually more than 90% at 5 μM. Whereas cell survival will reduce to 76% and 85% for INR1 and INR2,respectively ,while the concentration is increase to 10 μM. The result show that the probe possesses low toxicity for living cell under the condition just mentioned above. |

金蟲 (正式寫手)

金蟲 (正式寫手)

銅蟲 (小有名氣)
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實(shí)際應(yīng)用的理想細(xì)胞探針應(yīng)該最小擾動(dòng)生物系統(tǒng)的濃度。因此,采用MTT法測(cè)定細(xì)胞毒性和溶劑inr1 INR2。在一般情況下,在5μM化合物的濃度,細(xì)胞存活率估計(jì)為大于90%,培養(yǎng)2小時(shí)后(圖三),高濃度(10μM)可導(dǎo)致細(xì)胞的存活率下降76%和85%,inr1 INR2。結(jié)果表明,探針總體呈現(xiàn)低毒性的活細(xì)胞成像條件下的應(yīng)用。 An ideal cellular probe which used in practice should minimize the disturbance to the concentration of biological systems, therefore, we applied MTT to measure cytotoxicity as well as solvent INR1 and INR2. In general, at 5 μM concentration of a compound , cellular viability was estimated as greater than 90%, however, it will decreased to 76% to 85% for solvent INR1 and INR2 because of the high concentration(10μM) after 2h incubation (fig.3). The result indicates that the probes generally present under the condition of low toxicity for living cell imaging. |

鐵桿木蟲 (著名寫手)

銅蟲 (小有名氣)
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