| 5 | 1/1 | 返回列表 |
| 查看: 3240 | 回復(fù): 6 | ||||
| 本帖產(chǎn)生 1 個 MolEPI ,點(diǎn)擊這里進(jìn)行查看 | ||||
| 當(dāng)前只顯示滿足指定條件的回帖,點(diǎn)擊這里查看本話題的所有回帖 | ||||
mengxibio新蟲 (小有名氣)
|
[交流]
熒光PCR體系中是否需要加BSA 已有4人參與
|
|||
|
我們實(shí)驗(yàn)室?guī)缀醭闪藘膳桑环秸f應(yīng)該加,可以抵抗腐殖質(zhì),另一部分說不需要,而且兩方有時實(shí)驗(yàn)不同,效果各異。 大家平時做熒光pcr時是否添加bsa |
【分子生物】EPI帖 |
木蟲 (正式寫手)
|
BSA will coat the tube walls, preventing the template from being absorbed. This will reduce the frequency of primer dimers that occurs when the template concentration is low. BSA is also used to reduce the inhibition caused by humic acid contaminants. 源文檔 <http://www.protocol-online.org/biology-forums/posts/6051.html> Bovine Serum Albumin (BSA) is ideal for stabilization of enzymes during storage and for enzymatic reactions where the absence of nucleases is essential. BSA increases PCR yields from low purity templates. It also prevents adhesion of enzymes to the reaction tubes and tip surfaces. 源文檔 <http://www.fermentas.com/en/prod ... rs-reagents/b14-bsa>[ Last edited by gwbk on 2011-8-9 at 20:54 ] |
鐵桿木蟲 (職業(yè)作家)


鐵桿木蟲 (小有名氣)
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 301求調(diào)劑 +10 | yy要上岸呀 2026-03-17 | 10/500 |
|
|---|---|---|---|---|
|
[考研] 307求調(diào)劑 +10 | 冷笙123 2026-03-17 | 10/500 |
|
|
[考研] 085700資源與環(huán)境308求調(diào)劑 +12 | 墨墨漠 2026-03-18 | 13/650 |
|
|
[考研] 材料 336 求調(diào)劑 +3 | An@. 2026-03-18 | 4/200 |
|
|
[考研] 280求調(diào)劑 +7 | 咕嚕曉曉 2026-03-18 | 8/400 |
|
|
[考研] 一志愿武理材料305分求調(diào)劑 +6 | 想上岸的鯉魚 2026-03-18 | 7/350 |
|
|
[考研] 311求調(diào)劑 +5 | 冬十三 2026-03-18 | 5/250 |
|
|
[考研] 274求調(diào)劑 +10 | S.H1 2026-03-18 | 10/500 |
|
|
[考研] 一志愿中海洋材料工程專碩330分求調(diào)劑 +8 | 小材化本科 2026-03-18 | 8/400 |
|
|
[考研] 藥學(xué)383 求調(diào)劑 +3 | 藥學(xué)chy 2026-03-15 | 5/250 |
|
|
[考研] 329求調(diào)劑 +9 | 想上學(xué)吖吖 2026-03-19 | 9/450 |
|
|
[考研] 中南大學(xué)化學(xué)學(xué)碩337求調(diào)劑 +3 | niko- 2026-03-19 | 6/300 |
|
|
[考研] 353求調(diào)劑 +3 | 拉鉤不許變 2026-03-20 | 3/150 |
|
|
[考研] 319求調(diào)劑 +3 | 小力氣珂珂 2026-03-20 | 3/150 |
|
|
[考研] 一志愿南理工085701環(huán)境302求調(diào)劑院校 +3 | 葵梓衛(wèi)隊 2026-03-20 | 3/150 |
|
|
[考研] 08工學(xué)調(diào)劑 +5 | 用戶573181 2026-03-20 | 5/250 |
|
|
[考研] 梁成偉老師課題組歡迎你的加入 +9 | 一鴨鴨喲 2026-03-14 | 11/550 |
|
|
[考研] 材料與化工求調(diào)劑 +7 | 為學(xué)666 2026-03-16 | 7/350 |
|
|
[考研] 326求調(diào)劑 +5 | 上岸的小葡 2026-03-15 | 6/300 |
|
|
[考研] 一志愿211 0703方向310分求調(diào)劑 +3 | 努力奮斗112 2026-03-15 | 3/150 |
|