| 24小時(shí)熱門(mén)版塊排行榜 |
| 7 | 1/1 | 返回列表 |
| 查看: 3239 | 回復(fù): 6 | ||||
| 本帖產(chǎn)生 1 個(gè) MolEPI ,點(diǎn)擊這里進(jìn)行查看 | ||||
mengxibio新蟲(chóng) (小有名氣)
|
[交流]
熒光PCR體系中是否需要加BSA 已有4人參與
|
|||
|
我們實(shí)驗(yàn)室?guī)缀醭闪藘膳桑环秸f(shuō)應(yīng)該加,可以抵抗腐殖質(zhì),另一部分說(shuō)不需要,而且兩方有時(shí)實(shí)驗(yàn)不同,效果各異。 大家平時(shí)做熒光pcr時(shí)是否添加bsa |
【分子生物】EPI帖 |
鐵桿木蟲(chóng) (職業(yè)作家)

木蟲(chóng) (正式寫(xiě)手)
|
BSA will coat the tube walls, preventing the template from being absorbed. This will reduce the frequency of primer dimers that occurs when the template concentration is low. BSA is also used to reduce the inhibition caused by humic acid contaminants. 源文檔 <http://www.protocol-online.org/biology-forums/posts/6051.html> Bovine Serum Albumin (BSA) is ideal for stabilization of enzymes during storage and for enzymatic reactions where the absence of nucleases is essential. BSA increases PCR yields from low purity templates. It also prevents adhesion of enzymes to the reaction tubes and tip surfaces. 源文檔 <http://www.fermentas.com/en/prod ... rs-reagents/b14-bsa>[ Last edited by gwbk on 2011-8-9 at 20:54 ] |

鐵桿木蟲(chóng) (小有名氣)

鐵桿木蟲(chóng) (小有名氣)
| 7 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿 西北大學(xué) ,070300化學(xué)學(xué)碩,總分287,雙非一本,求調(diào)劑。 +3 | 晨昏線(xiàn)與星海 2026-03-18 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 材料專(zhuān)碩英一數(shù)二306 +7 | z1z2z3879 2026-03-18 | 7/350 |
|
|
[考研]
|
然11 2026-03-19 | 4/200 |
|
|
[考研] 一志愿中南化學(xué)(0703)總分337求調(diào)劑 +8 | niko- 2026-03-19 | 9/450 |
|
|
[考研] 求調(diào)劑一志愿南京航空航天大學(xué)289分 +3 | @taotao 2026-03-19 | 3/150 |
|
|
[考研] 材料學(xué)求調(diào)劑 +4 | Stella_Yao 2026-03-20 | 4/200 |
|
|
[考博] 招收博士1-2人 +3 | QGZDSYS 2026-03-18 | 3/150 |
|
|
[考研] 求調(diào)劑 +3 | 暗涌afhb 2026-03-16 | 3/150 |
|
|
[考研] 一志愿中國(guó)海洋大學(xué),生物學(xué),301分,求調(diào)劑 +5 | 1孫悟空 2026-03-17 | 6/300 |
|
|
[考研] 081700化工學(xué)碩調(diào)劑 +3 | 【1】 2026-03-16 | 3/150 |
|
|
[考研] 0703化學(xué)調(diào)劑 +10 | 妮妮ninicgb 2026-03-15 | 14/700 |
|
|
[考研] 材料與化工求調(diào)劑 +7 | 為學(xué)666 2026-03-16 | 7/350 |
|
|
[考研] 346求調(diào)劑[0856] +3 | WayneLim327 2026-03-16 | 6/300 |
|
|
[考研] 一志愿985,本科211,0817化學(xué)工程與技術(shù)319求調(diào)劑 +10 | Liwangman 2026-03-15 | 10/500 |
|
|
[考研] 328求調(diào)劑,英語(yǔ)六級(jí)551,有科研經(jīng)歷 +3 | 生物工程調(diào)劑 2026-03-17 | 7/350 |
|
|
[考研] 材料工程專(zhuān)碩274一志愿211求調(diào)劑 +6 | 薛云鵬 2026-03-15 | 6/300 |
|
|
[考研] 機(jī)械專(zhuān)碩325,尋找調(diào)劑院校 +3 | y9999 2026-03-15 | 5/250 |
|
|
[考研] 070305求調(diào)劑 +3 | mlpqaz03 2026-03-14 | 4/200 |
|
|
[考研] 085601材料工程315分求調(diào)劑 +3 | yang_0104 2026-03-15 | 3/150 |
|
|
[考研] 288求調(diào)劑 +4 | 奇點(diǎn)0314 2026-03-14 | 4/200 |
|