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mengxibio新蟲(chóng) (小有名氣)
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[交流]
熒光PCR體系中是否需要加BSA 已有4人參與
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我們實(shí)驗(yàn)室?guī)缀醭闪藘膳桑环秸f(shuō)應(yīng)該加,可以抵抗腐殖質(zhì),另一部分說(shuō)不需要,而且兩方有時(shí)實(shí)驗(yàn)不同,效果各異。 大家平時(shí)做熒光pcr時(shí)是否添加bsa |
【分子生物】EPI帖 |
鐵桿木蟲(chóng) (小有名氣)
鐵桿木蟲(chóng) (職業(yè)作家)

木蟲(chóng) (正式寫(xiě)手)
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BSA will coat the tube walls, preventing the template from being absorbed. This will reduce the frequency of primer dimers that occurs when the template concentration is low. BSA is also used to reduce the inhibition caused by humic acid contaminants. 源文檔 <http://www.protocol-online.org/biology-forums/posts/6051.html> Bovine Serum Albumin (BSA) is ideal for stabilization of enzymes during storage and for enzymatic reactions where the absence of nucleases is essential. BSA increases PCR yields from low purity templates. It also prevents adhesion of enzymes to the reaction tubes and tip surfaces. 源文檔 <http://www.fermentas.com/en/prod ... rs-reagents/b14-bsa>[ Last edited by gwbk on 2011-8-9 at 20:54 ] |

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